All Photos(1)

C75

from human colon, 12022907

Synonym(s):
C-75, C 75

biological source

human colon

growth mode

Adherent

karyotype

Not specified

morphology

Epithelial

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

Related Categories

Cell Line Origin

Human colorectal adenocarcinoma, moderately well differentiated, Dukes′ stage D

Cell Line Description

The C75 cell line was established from a 56-year old male patient with a moderately well differentiated adenocarcnoma of the sigmoid colon classified as Dukes′ stage D

DNA Profile

STR-PCR Data:
Amelogenin: X,Y
CSF1PO: 12
D13S317: 10,13
D16S539: 8,11
D5S818: 12
D7S820: 9,11
THO1: 8,9.3
TPOX: 10
vWA: 17

Culture Medium

Iscove′s Modified Dulbecco′s Medium, + 10% Foetal Bovine Serum (FBS) + 2 mM Glutamine

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37 °C. C75 cells grow very slowly (see growth curve); following resuscitation or subculture the cells take at least 48 hours.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Disclaimer

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.

Certificate of Analysis

Certificate of Origin

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service