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C70 cell line human

12022903

Synonym(s):
C-70, C 70

biological source

human colon

growth mode

adherent

morphology

epithelial

shipped in

dry ice

storage temp.

−196°C

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Cell Line Description

The C70 cell line was established from a 60-year old female patient with a moderately well differentiated adenocarcinoma of the sigmoid colon classified as Dukes′ stage B.

DNA Profile

STR-PCR Data:

Amelogenin: X
CSF1PO: 12
D13S317: 12
D16S539: 11,12
D5S818: 10
D7S820: 9
THO1: 6,9.3
TPOX: 8,11
vWA: 17,18

Culture Medium

Iscove′s Modified Dulbecco′s Medium, + 10% Fetal Bovine Serum (FBS) + 2 mM Glutamine

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4 x 10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37°C.

C70 cells grow very slowly (see growth curve); following resuscitation or subculture the cells take at least 48 hours to re-attach. Cells should be left without disturbance during this time to facilitate adhesion. Centrifugation of the cells (100g x 5 min) at resuscitation to remove DMSO improves the establishment of a viable culture. Once attached, the cells grow in discrete islands and use of trypsin or trypsin/EDTA to subculture the cells (even without knocking the flask) yields large clumps. Further disaggregation may be achieved by repeatedly pipetting the cells.

Other Notes

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Disclaimer

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.

Certificate of Analysis

Certificate of Origin

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