Cell Line Description
The C84 cell line was established from a 67-year old male patient with a poorly differentiated adenocarcinoma of the caecum classified as Dukes′ stage C.
Iscove′s Modified Dulbecco′s Medium, + 10% Fetal Bovine Serum (FBS) + 2 mM Glutamine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4 x 10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37°C.
C84 cells grow very slowly; following resuscitation or subculture the cells take at least 48 hours to re-attach. Cells should be left without disturbance during this time to facilitate adhesion. Centrifugation of the cells (100g x 5 min) at resuscitation to remove DMSO improves the establishment of a viable culture. Once attached, the cells grow in discrete islands and use of trypsin or trypsin/EDTA to subculture the cells (even without knocking the flask) yields large clumps. Further disaggregation may be achieved by repeatedly pipetting the cells.
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This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.