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C10

from human colon, 12022901

Synonym(s):
C 10, C-10

biological source

human colon

growth mode

Adherent

karyotype

Not specified

morphology

Epithelial

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Related Categories

Cell Line Origin

Human colorectal adenocarcinoma, moderately well differentiated, Dukes′ stage B

Cell Line Description

The C10 cell line was established from a 71-year old male patient with moderately well differentiated adenocarcinoma of the descending colon classified as Dukes′ stage B.

DNA Profile

STR-PCR Data:
Amelogenin: X,Y
CSF1PO: 11
D13S317: 13
D16S539: 11
D5S818: 12
D7S820: 10,13
THO1: 6,9.3
TPOX: 8,9
vWA: 14,15

Culture Medium

Iscove′s Modified Dulbecco′s Medium, + 10% Fetal Bovine Serum (FBS) + 2 mM Glutamine

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6, i.e., seeding at 2-4x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37 °C.

C10 cells grow very slowly (see growth curve); following resuscitation or subculture the cells take at least 48 hours to re-attach. Cells should be left without disturbance during this time to facilitate adhesion. Centrifugation of the cells (100 g x 5 min) at resuscitation to remove DMSO improves the establishment of a viable culture. Once attached, the cells grow in discrete islands and use of trypsin or trypsin/EDTA to subculture the cells (even without knocking the flask) yields large clumps. Further disaggregation may be achieved by repeatedly pipetting the cells.

Other Notes

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Disclaimer

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.

Certificate of Analysis

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Certificate of Origin

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