Cell Line Origin
Colorectal carcinoma from human colon
Human colorectal carcinoma
For complete product information, please see ECACC
RPMI1640 (with 2mM L-Glutamine+25mM HEPES) + 10% FCS, Insulin 0.6?g/ml, Hydrocortisone 1μg/ml, 1-Thioglycerol 10 μM
Optimal split ratio 1:2-1:4 (seeding density 2 x104 cells/cm2).Cells incubated at 37°C with 5% CO2.Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 min.
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
Depositor and originator: Tony Burgess - Ludwig Institute for Cancer Research, Australia