All Photos(1)
biological source
Mouse x Rat Glioma Hybrid brain
growth mode
Adherent
karyotype
Not specified
morphology
Neuronal
products
Acetylcholine and noradrenaline
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Related Categories
Cell Line Origin
Mouse neuroblastoma x rat glioma hybrid
Cell Line Description
NH15-CA2 is a hybrid cell line formed by the fusion of N115-BU-8 (a bromodeoxyuridine-resistant mutant of N1E-115 (Sigma Catalogue number 08062519) and 108CC15 (Sigma Catalogue number 08062516) (itself a mouse neuroblastoma N18TG2 x rat glioma C6-BU-1 hybrid line). This “hybrid-hybrid” line contains both cholinergic and adrenergic enzyme activities.
Application
used as a neuronal model
Culture Medium
DMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS) HAT (0.1 mM hypoxanthine, 10 μM aminopterin and 16 μM thymidine) Cells can be cultured without HAT provided they were cultured inbetween in HT medium for 3-4 days
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-2x10,000 cells/cm2 using 0.05% trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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Certificate of Analysis
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Certificate of Origin
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