All Photos(1)

H157

07030901

biological source

human mouth

growth mode

Adherent

karyotype

Not specified

morphology

Polygonal

products

Not specified

receptors

TGFbeta type I receptor, TGFbeta II receptor

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Related Categories

Cell Line Origin

Human oral squamous cell carcinoma

Cell Line Description

Established from a squamous cell carcinoma (SCC) of the buccal mucosa (20mm-40mm) of a male patient, age 84. STNMP satge II, well differentiated, node positive tumour. Mutant p53, codon 306 exon 8, G to A; wild type K-, N- and Ha-ras. Non-tumourigenic in athymic nude mice by subcutaneous injection and on injection into the floor of the mouth (orthotopic), but form epidermoid cysts subcutaneously.

DNA Profile

STR-PCR Data:

Amelogenin: X,Y
CSF1PO: 10
D13S317: 8,12
D16S539: 11
D5S818: 10,11
D7S820: 9
THO1: 9.3
TPOX: 8
vWA: 16

Culture Medium

DMEM:HAMS F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 0.5 ug/ml sodium hydrocortisone succinate

Subculture Routine

Split sub-confluent cultures (70-80%) 1:8 to 1:10 5% CO2; 37°C. Suggested seeding density 5 x 1000 cells/cm2.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Disclaimer

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.

Certificate of Analysis

Certificate of Origin

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service