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KARPAS 422

06101702, from human lymph(lymphoma)

Synonym(s):
KARPAS, 422

biological source

human lymph (lymphoma)

growth mode

Suspension

karyotype

Hyperdiploid with 10% polyploidy

morphology

Round polygonal cells growing singly or in small clusters in suspension

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

Related Categories

Cell Line Origin

Human B cell non-Hodgkin lymphoma

Cell Line Description

Established from a pleural effusion of 73 year-old woman diagnosed with B-cell non-Hodgkin lymphoma (intra-abdominal, diffuse large cell lymphoma, refractory, terminal). Carries t(14;18) IGH-BCL2 fusion gene (break point in major breakpoint region, MBR).

DNA Profile

Not specified

Culture Medium

RPMI 1640 + 2 mM Glutamine + 20% Fetal Bovine Serum (FBS).

Subculture Routine

Maintain cultures between 0.5 - 2 x 1000,000 cells/ml; 5% CO2; 37 °C. Doubling time approximately 60-90 hours. Split saturated culture 1:2 every 2-4 days. Round polygonal cells growing singly or in small clusters in suspension.

Other Notes

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Disclaimer

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.

Certificate of Analysis

Certificate of Origin

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