Cell Line Origin
Human B cell non-Hodgkin lymphoma
Cell Line Description
Established from a pleural effusion of 73 year-old woman diagnosed with B-cell non-Hodgkin lymphoma (intra-abdominal, diffuse large cell lymphoma, refractory, terminal). Carries t(14;18) IGH-BCL2 fusion gene (break point in major breakpoint region, MBR).
RPMI 1640 + 2 mM Glutamine + 20% Fetal Bovine Serum (FBS).
Maintain cultures between 0.5 - 2 x 1000,000 cells/ml; 5% CO2; 37 °C. Doubling time approximately 60-90 hours. Split saturated culture 1:2 every 2-4 days. Round polygonal cells growing singly or in small clusters in suspension.
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This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.