H400

06092006

Properties

• biological source: human mouth
• growth mode: Adherent
• karyotype: Not specified
• morphology: Polygonal
• products: Not specified
• receptors: TGFbeta type I receptor, TGFbeta II receptor, TGFbeta III receptor
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human oral squamous cell carcinoma, alveolar process

Cell Line Description

Established from a squamous cell carcinoma (SCC) of the alveolar process (20mm - 40mm) of a female patient aged 55. STNMP stage II, moderately differentiated, node negative tumour. Mutant p53, codon 283 exon 8, C to G wild type K-, N- and Ha-ras. This cell line is highly responsive to TGFbeta. These cells are non-tumourigenic on subcutaneous injection into athymic nude mice, but tumourigenic on injection into the floor of the mouth. Haplotype information: A*11,A*29; B*07,B*15; Cw*03,Cw*15

Culture Medium

DMEM:HAMS F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 0.5 ug/ml sodium hydrocortisone succinate

Subculture Routine

Split sub-confluent cultures (70-80%) 1:8 to 1:10 using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Suggested seeding density 5 x 1000 cells/cm². Cells can take approximately 10 minutes to detach, an alternative is to trypsinise 2 to 3 times with fresh trypsin for shorter periods for each trypsin application. Avoid knocking flasks during the trypsinisation process as this can lead to loss of viability.

Other Notes

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Disclaimer

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.