H376

06092005

Properties

• biological source: human mouth
• growth mode: Adherent
• karyotype: Not specified
• morphology: Polygonal
• products: Not specified
• receptors: TGFbeta type I receptor, TGFbeta II receptor, TGFbeta III receptor
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human oral squamous cell carcinoma, floor of mouth

Cell Line Description

Established from a squamous cell carcinoma (SCC) of the floor of the mouth (20 mm-40 mm) of a 40 year-old female patient. STNMP stage III, well differentiated, node positive tumour. Mutant p53, codon 266 exon 8, G to T; wild type K-, N-, and Ha-ras. This cell line is highly responsive to TGF (Transforming growth factor )-β. H376 cells in culture scatter, i.e., the cells redistribute on the substrate, in response to Hepatocyte Growth Factor. Non-tumourigenic in athymic nude mice by subcutaneous injection and on injection into the floor of the mouth (orthotopic). Haplotype information: A*03,A*24; B*27,B*35; Cw*04,Cw*12

DNA Profile

STR-PCR Data:
Amelogenin: X
CSF1PO: 10,11
D13S317: 8,11
D16S539: 11
D5S818: 10,12
D7S820: 8,10
THO1: 6,9
TPOX: 8
vWA: 16,18

Culture Medium

DMEM:HAMS F12 (1:1) + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS) + 0.5 ug/ml sodium hydrocortisone succinate.

Subculture Routine

Split sub-confluent cultures (70-80%), approximately every 5-6 days, 1:8 to 1:10 using 0.25% trypsin/EDTA; 5% CO₂; 37 °C. Suggested seeding density 5 x 1000 cells/cm².

Other Notes

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Disclaimer

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.