Cell Line Origin
Human oral squamous cell carcinoma, floor of mouth
Cell Line Description
Established from a squamous cell carcinoma (SCC) of the floor of the mouth (20 mm-40 mm) of a 40 year-old female patient. STNMP stage III, well differentiated, node positive tumour. Mutant p53, codon 266 exon 8, G to T; wild type K-, N-, and Ha-ras. This cell line is highly responsive to TGF (Transforming growth factor )-β. H376 cells in culture scatter, i.e., the cells redistribute on the substrate, in response to Hepatocyte Growth Factor. Non-tumourigenic in athymic nude mice by subcutaneous injection and on injection into the floor of the mouth (orthotopic). Haplotype information: A*03,A*24; B*27,B*35; Cw*04,Cw*12
DMEM:HAMS F12 (1:1) + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS) + 0.5 ug/ml sodium hydrocortisone succinate.
Split sub-confluent cultures (70-80%), approximately every 5-6 days, 1:8 to 1:10 using 0.25% trypsin/EDTA; 5% CO2; 37 °C. Suggested seeding density 5 x 1000 cells/cm2.
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.