Cell Line Origin
Human oral squamous cell carcinoma, tongue
Cell Line Description
Established from a small squamous cell carcinoma (SSC) of the tongue (<20mm in size) of a 32 year-old male patient. STNMP stage 1, well differentiated, node negative tumour. Aneuploid- dual G-/G1 peak on cell cycle analysis by flow cytometry. Mutant p53, codon 244 exon 7; G to T; wild type K-, N- and Ha-ras. Tumourigenic in athymic nude mice, both by subcutaneous injection and injection into the floor of the mouth. By short tandem repeat (STR)-PCR analysis the Y chromosome could not be detected in this cell line when tested at ECACC. It is a known phenomenon that SSC cell lines can lose their Y chromosome.
DMEM:HAMS F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 0.5 ug/ml sodium hydrocortisone succinate
Split sub-confluent cultures (70-80%) 1:8 to 1:10 using 0.25% trypsin/EDTA; 5% CO2; 37°C. Suggested seeding density 5 x 1000 cells/cm2. Cells can take approximately 10 minutes to detach, an alternative is to trypsinise 2 to 3 times with fresh trypsin for shorter periods for each trypsin application. Avoid knocking flasks during the trypsinisation process as this can lead to loss of viability.
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This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.