Cell Line Origin
Tongue squamous cell carcinoma
Cell Line Description
Adherent keratinocyte cell line derived from a squamous cell carcinoma of the tongue of a Caucasian female. Keratin and involucrin markers present. Known mutations: p53 and p16. Cells are tumourigenic in athymic mice. Epidermal Growth Factor Receptor (EGFR) present.
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 0.4 micrograms/ml Hydrocortisone.
Split sub-confluent cultures (70-80%) 1:2 - 1:4 using 0.25% trypsin/EDTA; 8% CO2; 37 C. Suggested seeding density 4 x 10,000 cells/cm2. A feeder layer of lethally-irradiated or mitomycin C-treated 3T3 feeder cells can be used to culture this cell line,
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This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.