All Photos(1)

BICR 16

06031001

biological source

human mouth

growth mode

Adherent

karyotype

Aneuploid

morphology

Epithelial, keratinocyte

products

Not specified

receptors

Epidermal Growth Factor Receptor (EGFR)

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Related Categories

Cell Line Origin

Tongue squamous carcinoma

Cell Line Description

Adherent cell line derived from a recurrent squamous cell carcinoma of the tongue of a Caucasian male. Keratin and involucrin markers present. Known mutations: p53, p16 and p14ARF. Cells are tumourigenic in athymic mice.

Culture Medium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 0.4 micrograms/ml Hydrocortisone.

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 using 0.25% trypsin/EDTA; 8% CO2; 37°C. Suggested seeding density 2-4 x 10,000 cells/cm2. A feeder layer of lethally-irradiated or mitomycin C-treated 3T3 feeder cells can be used to culture this cell line, howev

Other Notes

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Disclaimer

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.

Certificate of Analysis

Certificate of Origin

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