MilliporeSigma
All Photos(1)

BICR 31

04072107

Synonym(s):
BICR31 Cells, Beatson Institute for Cancer Research 31 Cells

biological source

human mouth

growth mode

Adherent

karyotype

Pseudodiploid/Aneuploid

morphology

Epithelial, keratinocyte

products

Not specified

receptors

Epidermal Growth Factor Receptor (EGFR)

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Tongue squamous carcinoma

Cell Line Description

Adherent cell line derived from a squamous cell carcinoma of the tongue of a Caucasian male. Keratin and involucrin markers present. Known mutations: p53, p16 and p14ARF. Cells are tumourigenic in athymic mice.

Application

Study of genotype/phenotype relationships

Culture Medium

DMEM + 2mM Glutamine + 2% Foetal Bovine Serum (FBS) + 0.4 micrograms/ml Hydrocortisone.

Subculture Routine

Split sub-confluent cultures (70-80%) 1:10 - 1:50 using 0.25% trypsin/EDTA; 8% CO2; 37 °C. Suggested seeding density 2 x 10,000 cells/cm2.

Culture cells on a feeder layer of lethally-irradiated or mitomycin C-treated 3T3 Swiss Albino cells (Sigma catalog number 85022108). Feeder layers are prepared in the flasks at least 24 h in advance of being required. Where frozen stocks of treated 3T3 Swiss Albino cells have been prepared, an ampoule is thawed in 37 °C water bath and the contents quickly transferred to a 15 ml centrifuge tube. DMEM medium is added drop wise to 5 ml. Cells are centrifuged at 150 x g for 5 min at room temperature. Cells are resuspended in 5 ml of medium. Cells are counted and added to flasks containing the correct BICR growth medium at 1-3 x 104 cells/cm2.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Disclaimer

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service