All Photos(2)

C9426

Sigma-Aldrich

CHAPS hydrate

BioReagent, suitable for electrophoresis, ≥98% (HPLC)

Synonym(s):
3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate hydrate, CHAPS
Empirical Formula (Hill Notation):
C32H58N2O7S · xH2O
CAS Number:
Molecular Weight:
614.88 (anhydrous basis)
PubChem Substance ID:
NACRES:
NB.22

Quality Level

description

zwitterionic

product line

BioReagent

assay

≥98% (HPLC)

form

powder

mol wt

614.88 g/mol (anhydrous basis)

technique(s)

electrophoresis: suitable
protein quantification: suitable

impurities

<0.04% DMF

mp

157 °C (dec.) (lit.)

solubility

water: 50 mg/mL, clear, colorless

suitability

suitable for electrophoresis

storage temp.

room temp

SMILES string

O.C[C@H](CCC(=O)NCCC[N+](C)(C)CCCS([O-])(=O)=O)[C@H]1CC[C@H]2[C@@H]3[C@H](O)C[C@@H]4C[C@H](O)CC[C@]4(C)[C@H]3C[C@H](O)[C@]12C

InChI

1S/C32H58N2O7S.H2O/c1-21(8-11-29(38)33-14-6-15-34(4,5)16-7-17-42(39,40)41)24-9-10-25-30-26(20-28(37)32(24,25)3)31(2)13-12-23(35)18-22(31)19-27(30)36;/h21-28,30,35-37H,6-20H2,1-5H3,(H-,33,38,39,40,41);1H2/t21-,22+,23-,24-,25+,26+,27-,28+,30+,31+,32-;/m1./s1

InChI key

SJCUTFKCLFLIFE-JWTJKVBLSA-N

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General description

CHAPS is a non-denaturing, zwitterionic sulfobetaine detergent. It is mild and solubilizes membrane proteins and receptors. It is useful for breaking protein-protein interactions. It is a derivative of the naturally occurring bile salts. It has small micellar molecular weight (6,150) and high critical micellar concentration (6 - 10 mM) allowing it to be removed from samples by dialysis.

Application

CHAPS has been used:
  • For membrane permeabilization of cells
  • As a component in lysis buffer for homogenization of liver tissues as well as adipose tissue
  • As a component in denaturing buffer in enzyme characterization
  • As a component of 2D- sample buffer in 2D electrophoresis
  • For protein solubilization in isoelectric focusing

Packaging

1, 5 g in glass bottle

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Repr. 1B

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Product Information Sheet

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Non-autophagic GFP-LC3 puncta induced by saponin and other detergents
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Autophagy, 3(6), 586-590 (2007)
Effect of copper nanoparticles exposure in the physiology of the common carp (Cyprinus carpio): Biochemical, histological and proteomic approaches.
Gupta Y, et al.
Aquaculture and Fisheries, 1, 15-23 (2016)
Investigation of protein-tyrosine phosphatases by in-gel assays.
Markova B, et al.
Methods, 35(1), 22-27 (2005)
Dependence of critical micelle concentration of a zwitterionic detergent on ionic strength: implications in receptor solubilization.
Chattopadhyay A and Harikumar KG
Febs Letters, 391(1-2), 199-202 (1996)

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