CD44 is a glycosylated cartilage-linked protein. The protein exists in a variety of forms with different molecular structures, ranging from 85 kDa to 250 kDa. It is a multistructural and multifunctional cell surface molecule and involved in various biological processes such as cell proliferation, cell differentiation, cell migration, angiogenesis, presentation of cytokines, chemokines, and growth factors to the corresponding receptors, and docking of proteases at the cell membrane, as well as in signaling for cell survival. Although all the activities are related to normal cell, but it has also some pathologic activities of cancer cells.
Recognizes the CD44 (80-95 kDa) human cell surface glycoprotein on B and T lymphocytes, monocytes, granulocytes and red blood cells. The epitope recognized by this antibody is sensitive to bromelain and other proteolytic enzymes. It is detectable in routine formalin-fixed, paraffin-embedded tissue.
5th Workshop: code no. BP406, S320
circulating malignant human Sezary T cells.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Flow cytometry/Cell sorting (1 paper)
Monoclonal Anti-CD44 antibody was used as a hyaluronic acid receptor for indirect immunoperoxidase technique in a study to evaluate the relationship of lymphoid cells and macrophages with vasculature and stromal components.
Anti CD44 monoclonal antibody was used for:
- immunostaining of nonpapillary carcinoma fine-needle aspiration (FNA) or surgical excision specimen.
- flow cytometry analysis to assess breast CSCs in human MCF-7/Dox breast cancer cells.
- staining of CD44 in immunohistochemistry analysis.
- incubation of osteoclasts (OCs) in a study to analyze the molecular composition and F-actin organization of the OC podosome belts.
- It is also suitable for western blot at a dilution of 1:500-1:2,000
The CD44 antigen is expressed on a variety of cell types including peripheral blood leukocytes (B and T lymphocytes, monocytes, granuloctes) and red cells. It is also weakly expressed on platelets. The antibody is also reactive with bone marrow nucleated cells, medullary thymocytes, liver Kupffer cells, fibroblasts, corneal cells, epidermal keratinocytes, synovial cells, a subset of pancreatic acinar cells and brain cells. The epitope recognized by this clone is sensitive to formalin fixation and paraffin embedding.
The CD44 antigen has been studied under the following names: Pgp-1, Hermes antigen, ECM-III, H-CAM and HUTCH-1. It functions as a homing receptor. CD44 antigen is the cellular receptor for hyaluronic acid. The antibody is also reactive with bone marrow nucleated cells, medullary thymocytes, liver Kupffer cells, fibroblasts, corneal cells, epidermal keratinocytes, synovial cells, a subset of pancreatic acinar cells and brain cells.
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
Storage and Stability
Store at 2-8°C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
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