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C7886

Sigma-Aldrich

Creatine Phosphokinase from bovine heart

Type III, salt-free, lyophilized powder, ≥30 units/mg protein

Synonym(s):
ATP: Creatine N-Phosphotransferase, CPK, Creatine Kinase, Phosphocreatine phosphokinase
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
eCl@ss:
32160410
NACRES:
NA.54

biological source

bovine heart

Quality Level

description

Contains primarily isozyme MM

type

Type III

form

salt-free, lyophilized powder

specific activity

≥30 units/mg protein

mol wt

80 kDa

storage temp.

−20°C

Application

Creatine Phosphokinase from bovine heart has been used:
  • in the reaction mix for the import of in vitro synthesized wild-type FLAG/MYC-tagged LYR Motif Containing 7 (LYRM7-F/M) or the LFK tripeptide replaced with alanine (LFK-AAA) mutant into isolated mitochondria
  • in autoubiquitination assay
  • in the preparation of premix buffer for adenylyl cyclase activity
  • in the preparation of E-mix for in vitro nuclear assembly and isolation

Creatine phosphokinase from bovine heart has been used to investigate whether endothelial cell growth is stimulated by ischemic hearts. Creatine phosphokinase from bovine heart has also been used to evaluate the effect of high but nontoxic dietary intake of copper and selenium on metabolism in calves.
The product has been used for tATPase assay of myofibrillar protein isolated from rabbit. This assay evaluated the kinetic influence of bound creatine kinase (CK) on Ca2+-activated myosin ATPase. The product has also been used for the enzymatic hydrolysis of protein samples during tryptophan estimation by pyrolysis gas chromatography.

Packaging

500, 1000, 3500, 17500 units in poly bottle

Biochem/physiol Actions

Creatine kinase plays a key role in the energy metabolism of cells with intermittently high and fluctuating energy requirements. Examples of such cells include cardiac or skeletal muscle cells and neural tissues of brain and retina. The enzyme catalyzes the reversible transfer of the phosphoryl group from phosphorylcreatine to ADP, in order to generate ATP. The molecular mass of the protein is found to be approximately 80,000 Da. It is made up of 2 subunits, each having a molecular weight of 40,000 ± 2000. The lighter subunit is present in larger amounts.

Caution

The use of 0.1% albumin in the reaction buffer is recommended to avoid inactivation due to dilution.

Unit Definition

One unit will transfer 1.0 μmole of phosphate from phosphocreatine to ADP per min at pH 7.4 at 30 °C.

Analysis Note

Protein determined by biuret.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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