MilliporeSigma
All Photos(1)

A3937

Sigma-Aldrich

Anti-Rabbit IgG (whole molecule), F(ab′)2 fragment−Alkaline Phosphatase antibody produced in goat

affinity isolated antibody, buffered aqueous glycerol solution

MDL number:
NACRES:
NA.46

biological source

goat

Quality Level

conjugate

alkaline phosphatase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous glycerol solution

species reactivity

rabbit

technique(s)

direct ELISA: 1:30,000
dot blot: 1:30,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50
western blot: 1:30,000

shipped in

wet ice

storage temp.

2-8°C

Looking for similar products? Visit Product Comparison Guide

Related Categories

General description

IgGs are glycoprotein antibodies that modulate several immune responses. Rabbit IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rabbit IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins. Anti-Rabbit IgG (whole molecule), (F(ab′)2) fragment-Alkaline Phosphatase antibody binds all rabbit immunoglobulins.

Immunogen

Purified rabbit IgG

Application

Anti-Rabbit IgG (whole molecule), (F(ab′)2) fragment-Alkaline Phosphatase antibody is suitable for use in immunoblotting (5μl) and direct ELISA (1:30,000). The product may also be used for immunohistochemistry (1:50 using formalin-fixed, paraffin-embedded sections).
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Enzyme-linked immunosorbent assay (1 paper)
Western Blotting (1 paper)
Western blot analysis of prostate epithelial cell lysates was performed using alkaline phosphatase conjugated goat anti-rabbit F2 Fragment specific antibody as the secondary at a dilution of 1:7500 for 2 hours at room temperature.

Physical form

Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl2, 10 mM glycine, 1% bovine serum albumin, 50% glycerol and 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

SDS

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service