MilliporeSigma
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A3854

Sigma-Aldrich

Anti-β-Actin−Peroxidase antibody, Mouse monoclonal

clone AC-15, purified from hybridoma cell culture

Synonym(s):
Loading Control, Monoclonal Anti-β-Actin
NACRES:
NA.41

Quality Level

biological source

mouse

conjugate

peroxidase conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

AC-15, monoclonal

form

buffered aqueous solution

mol wt

antigen 42 kDa

species reactivity

pig, Hirudo medicinalis, bovine, rat, canine, feline, human, rabbit, carp, mouse, guinea pig, chicken, sheep

should not react with

Dictyostelium discoideum
Drosophila

technique(s)

western blot: 1:25,000-1:50,000 using cell extracts of human foreskin fibroblasts or chicken fibroblasts

isotype

IgG1

UniProt accession no.

application(s)

research pathology

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... ACTB(60)
mouse ... Actb(11461)
rat ... Actb(81822)

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General description

Mouse monoclonal anti-β-actin-peroxidase antibody specifically localizes β-actin in a wide variety of tissues and species using immunoblotting (42kDa). The antibody cross-reacts with β-Actin expressed in cells of human, bovine, sheep, pig, rabbit, cat, dog, mouse, rat, guinea pig, chicken, carp, and Hirudo medicinalis (leech) tissues, but not in Dictyostelium discoideum amoebae or Drosophila.
In staining of chicken gizzard ultrathin tissue cryosections, the antibody labels the dense bodies and longitudinal channels linking consecutive dense bodies that are also occupied by desmin and the membrane-associated dense plaque. It does not stain adult cardiac and skeletal muscles except for traces due to contaminations of the sample with non-muscle cells, or if embryonic tissue is being used.
Actin is one of the most conserved eukaryotic proteins and is expressed in mammals and birds as at least six isoforms. There are three α-actins (skeletal, cardiac, and smooth muscle), one β-actin (β-nonmuscle), and two γ-actins (γ-smooth muscle and γ-non muscle). Actin isoforms show >90% overall sequence homology, but only 50–60% homology in their NH2-terminal residues. ACTB (actin β) gene codes for β-actin. It is a cytoskeletal housekeeping protein. It is present in the cytoplasm. The ACTB gene is located on human chromosome 7p22.1.
Mouse monoclonal anti-β-actin-peroxidase antibody specifically localizes β-actin in a wide variety of tissues and species using immunoblotting (42kDa). The antibody cross-reacts with β-Actin expressed in cells of human, bovine, sheep, pig, rabbit, cat, dog, mouse, rat, guinea pig, chicken, carp, and Hirudo medicinalis (leech) tissues, but not in Dictyostelium discoideum amoebae or Drosophila.

Immunogen

slightly modified β-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH.

Application

Monoclonal mouse anti-actin was used as a loading control for western blot analysis of rat liver protein lysates.
Mouse monoclonal anti-β-actin-peroxidase antibody can be used as a primary antibody for western blot assays.
Monoclonal mouse anti-actin antibody was used as a loading control for western blot analysis of immunoprecipitated proteins from rat dorsal root ganglion cocultures.
Monoclonal mouse anti-actin antibody was used as a loading control for western blot analysis of immunoprecipitated proteins from rat dorsal root ganglion cocultures.
Monoclonal mouse anti-actin was used as a loading control for western blot analysis of rat liver protein lysates.
Mouse monoclonal anti-β-actin-peroxidase antibody can be used as a primary antibody for western blot assays. Monoclonal Anti-ß-Actin-Peroxidase antibody has been used in western blot analysis and chemiluminescent detection.

Biochem/physiol Actions

The two major cytoskeletal proteins implicated in cell motility are actin and myosin. Actin and myosin are constituents of many cell types and are involved in cellular process including locomotion, secretion, cytoplasmic streaming, phagocytosis, and cytokinesis. The six isoforms of actins are characterized by electrophoresis and amino acid sequence analysis. Four of them represent the differentiation markers of muscle tissues and two are found practically in all cells. β-Actin (ACTB) modulates cell growth, migration and the G-actin pool. Mutations in ACTB leads to pleiotropic developmental disorder.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For prolonged storage, freeze in working aliquots. Repeated freezing and thawing, or storage in frost-free freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Other Notes

To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Skin Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

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Quotes and Ordering

Ahmed Raafat et al.
Journal of cellular physiology, 226(7), 1940-1952 (2011-04-21)
Notch genes play a critical role in mammary gland growth, development and tumorigenesis. In the present study, we have quantitatively determined the levels and mRNA expression patterns of the Notch receptor genes, their ligands and target genes in the postnatal
Regulatory interplay between miR-21, JAG1 and 17beta-estradiol (E2) in breast cancer cells.
Selcuklu S D, et al.
Biochemical and Biophysical Research Communications, 423(2), 234-239 (2012)
Cezary Skobowiat et al.
American journal of physiology. Endocrinology and metabolism, 301(3), E484-E493 (2011-06-16)
The hypothalamic-pituitary-adrenal (HPA) axis maintains basal and stress-related homeostasis in vertebrates. Skin expresses all elements of the HPA axis including corticotropin-releasing hormone (CRH), proopiomelanocortin (POMC), ACTH, β-endorphin (β-END) with corresponding receptors, the glucocorticoidogenic pathway, and the glucocorticoid receptor (GR). To
Post-treatment with rapamycin does not prevent epileptogenesis in the amygdala stimulation model of temporal lobe epilepsy.
Anna S, et al.
Neuroscience Letters, 509(2), 105-109 (2012)
Georg Eschenburg et al.
Cancer research, 72(10), 2645-2656 (2012-04-12)
Despite intensive treatment regimens, high-risk and late-stage neuroblastoma tends to have a poor survival outcome. Overexpression of the apoptotic regulator, X-linked inhibitor of apoptosis protein (XIAP), has been associated with chemotherapy resistance in several cancers including neuroblastoma. Here, we report

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