Rabbit anti-ATG5 (N-terminal) recognizes human, rat, and mouse Atg5-Atg12 complex.
synthetic peptide corresponding to amino acids 2-15 of human ATG5, conjugated to KLH via a C-terminal cysteine residue. The corresponding sequence is identical in rat and mouse.
Rabbit anti-ATG5 has been used for immunofluorescence and chemiluminescent western blot analyses.
Rabbit anti-ATG5 (N-terminal specific) was used for western blot analysis to detect the level of ATG5 in SK-N-MC cells after transduction with an adenovirus expressing a shRNA specific to ATG5.
Anti-ATG5 (N-terminal) antibody has been used:
- in immunoblotting
- in immunofluorescence
- in western blotting
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Atg5 (Apg5) is a 32 kDa protein that is required for autophagy. Atg5 is covalently modified by Atg12, a ubiquitin-like modifier. The Atg12-Atg5-Atg16 complex thus formed, localizes to autophagosome precursors and regulates autophagosome formation. Studies have reported that Atg5 fragment that is formed by calpain cleavage has pro-apoptotic properties.
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide
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