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CMC0002

Sigma-Aldrich

SIG10 F′ Chemically Competent Cells

for protein expression and DNA plasmid production

grade

for molecular biology

form

buffered aqueous solution

cell transformation

competent cell type: chemically competent
transformation efficiency: ≥5 x 108 cfu/μg

shipped in

dry ice

storage temp.

−70°C

General description

An excellent choice for producing single-stranded DNA for sequencing, mutagenesis, etc.

Genotype

[F´ pro A+B+ lacIqZΔM15::Tn10 (TetR)] /mcrA Δ(mrr-hsdRMS-mcrBC) endA1 recA1 Φ80dlacZ?M15 ΔlacX74 araD139 Δ(ara, leu)7697 galU galK rpsL nupGλ tonA

Features and Benefits

  • share the most useful genetic elements of standard cloning strains like DH5α, DH10B JM109, TOP10, etc. and directly replace them in cloning protocols.
  • provide the performance researchers need with ease of use.
  • provide solutions for a wide range of applications at economical prices.
  • Blue - white screening

Components

  • SIG10 F′ chemically competent cells
  • pUC 19 transformation control DNA
  • recovery medium for cloning

Legal Information

DH10B is a trademark of Life Technologies
DH5α is a trademark of Invitrogen Corp.

Certificate of Analysis

Certificate of Origin

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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