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B4287

Sigma-Aldrich

Bovine Serum Albumin

heat shock fraction, protease free, suitable for hybridization, pH 7, ≥98%

Synonym(s):

Albumin bovine serum, BSA, Bovine albumin

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About This Item

CAS Number:
9048-46-8
EC Number:
232-936-2
MDL number:
MFCD00130384
UNSPSC Code:
12352202
NACRES:
NA.25

biological source

bovine

Quality Level

200

grade

for molecular biology

product line

BioReagent

assay

≥98%

form

lyophilized powder

mol wt

~66 kDa

purified by

heat shock fractionation

packaging

poly bottle of

origin

USA origin

technique(s)

ELISA: suitable
electrophoresis: suitable
western blot: suitable

pH

7

solubility

water: soluble (40 mg/ml)

UniProt accession no.

P02769

foreign activity

Protease free

storage temp.

2-8°C

Gene Information

bovine ... ALB(280717)

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Application

Select the right albumin for your application with the Albumin Selection Guide.
A non-acetylated protein suitable as a blocking agent in Southern blotting. If your application requires nuclease-free BSA, we recommend our acetylated BSA for these critical applications.

Biochem/physiol Actions

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Features and Benefits

  • Protease free
  • Suitable for blocking non-specific protein binding sites in hybridizations
  • Heat shock fractionated

Preparation Note

Prepared using heat shock fractionation
Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Journal of immunology (Baltimore, Md. : 1950), 181(7), 4883-4893 (2008-09-20)
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