All Photos(1)

93443

Sigma-Aldrich

Triton X-100 solution

BioUltra, for molecular biology, ~10% in H2O

Synonym(s):
4-(1,1,3,3-Tetramethylbutyl)phenyl-polyethylene glycol solution, Triton X-100, t-Octylphenoxypolyethoxyethanol, Polyethylene glycol tert-octylphenyl ether
Linear Formula:
t-Oct-C6H4-(OCH2CH2)xOH, x= 9-10
CAS Number:
Beilstein:
2315025
MDL number:
PubChem Substance ID:

Quality Level

grade

for molecular biology

description

non-ionic

product line

BioUltra

mol wt

micellar avg mol wt 80,000
average mol wt 625

concentration

~10% in H2O

aggregation number

100-155

impurities

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected

refractive index

n20/D 1.349±0.002

pH

6.5-8.5 (25 °C)

CMC

0.2-0.9 mM (20-25°C)
0.23 mM

transition temp

cloud point 65 °C
pour point ~7 °C

density

1.01 g/mL at 20 °C

cation traces

Al: ≤1 mg/kg
Ba: ≤1 mg/kg
Bi: ≤1 mg/kg
Ca: ≤5 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤1 mg/kg
Fe: ≤1 mg/kg
K: ≤100 mg/kg
Li: ≤1 mg/kg
Mg: ≤1 mg/kg
Mn: ≤1 mg/kg
Mo: ≤1 mg/kg
Na: ≤100 mg/kg
Ni: ≤1 mg/kg
Pb: ≤1 mg/kg
Sr: ≤1 mg/kg
Zn: ≤1 mg/kg

absorption

cut-off at 310 nm

HLB

13.5

SMILES string

CC(C)(C)CC(C)(C)c1ccc(OCCOCCOCCOCCOCCOCCOCCO)cc1

InChI

1S/C28H50O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h6-9,29H,10-24H2,1-5H3

InChI key

HNLXNOZHXNSSPN-UHFFFAOYSA-N

Looking for similar products? Visit Product Comparison Guide

Related Categories

General description

Triton X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection.

Application

Triton-X has been used:
  • for permeabilization of cells before techniques like indirect immunofluorescence and flow cytometry
  • As a component of lysis buffer used for homogenization of cells
  • As a component of blocking buffer in immunofluorescence staining
  • For the preparation of cells in double fluorescence staining

Biochem/physiol Actions

Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.

Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Pictograms

CorrosionEnvironment

Signal Word

Danger

Hazard Statements

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Skin Irrit. 2

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

483.8 °F - closed cup

Flash Point(C)

251 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

The effect of artificial shrinkage and assisted hatching on the development of mouse blastocysts and cell number after vitrification.
Kim H J, et al.
Clinical and Experimental Reproductive Medicine, 42(3), 94-100 (2015)
Creation of an injectable in situ gelling native extracellular matrix for nucleus pulposus tissue engineering.
Wachs R A, et al.
The Spine Journal : Official Journal of the North American Spine Society, 17(3), 435-444 (2017)
Oxysterols in adipose tissue-derived mesenchymal stem cell proliferation and death.
Silva S F, et al.
The Journal of Steroid Biochemistry and Molecular Biology, 169, 164-175 (2017)
Melittin exerts an antitumor effect on non small cell lung cancer cells.
Zhang S F and Chen Z
Molecular Medicine Reports, 16(3), 3581-3586 (2017)
Alena Balashova et al.
Frontiers in neuroscience, 13, 1033-1033 (2019-10-22)
It is well-known that hyaluronic acid (HA) as a component of brain extracellular matrix (ECM) plays a pivotal role in the nervous system and is involved in synaptic plasticity changes in vascular cognitive impairment and dementia. HA breakdown is a...

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service