COLO 699 N cells, a non-small cell lung cancer cell line is useful in subcutaneous xenografts studies to mimic in vivo model of human lung cancer. It finds applications in stable isotope-based proteome analysis.
Cell Line Origin
Human lung cancer
Cell Line Description
Derived from the pleural fluid of a 57 year old female with lung cancer. Please note: This cell line was found to be indistinguishable from cell lines RPMI 7932 & COLO 738 by STR PCR DNA profiling. At ECACC the STR PCR profile of all stocks of this cell line match the profile of the original source material received at ECACC. However, it is not clear if the cell lines COLO 699N, RPMI 7932 & COLO 738 were originally derived from the same individual as the profiles suggest. The cell lines COLO 699N, RPMI 7932 & COLO 738 were received from the same depositor. If we are able to obtain clarification of the relationship between these cell lines this information will be updated.
COLO 699 N has been used in cytotoxicity studies with Tegaran and 13-methyltetradecanoic acid (13-MTD).
STR-PCR Data: Amelogenin: X
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2using 0.25% trypsin/EDTA; 5% CO2; 37°C.
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