75633

Sigma-Aldrich

Osmium tetroxide solution

suitable for electron microscopy, 2% in H2O

Linear Formula:
OsO4
CAS Number:
Molecular Weight:
254.23
MDL number:
PubChem Substance ID:
NACRES:
NA.47

Quality Level

reaction suitability

reagent type: oxidant

concentration

2% in H2O

application(s)

electron microscopy: suitable
hematology: suitable
histology: suitable

color

colorless to yellow

density

1.0 g/mL at 20 °C
1.0 g/mL at 20 °C

storage temp.

2-8°C

SMILES string

O=[Os](=O)(=O)=O

InChI

1S/4O.Os

InChI key

VUVGYHUDAICLFK-UHFFFAOYSA-N

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Application

Osmium tetroxide solution has been used as a fixative in electron microscopy.

Biochem/physiol Actions

Osmium tetroxide is also referred to as osmium and is mainly used as a secondary fixative of tissues. The molecule reacts with lipid moieties and is responsible for the oxidation of unsaturated bonds of fatty acids. This reaction adds density and contrast to biological samples. In normal tissues, presence of osmium results in intense black staining which can be viewed by normal eye. In addition, it can be used as a mordant (a molecule which combines with stain or dye at later stages) in electron microscopy, thereby enhancing lead staining.

Pictograms

CorrosionSkull and crossbones

Signal Word

Danger

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

UN 3287 6.1 / PGII

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

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de Barros CM, et al.
Nitric Oxide, 38, 26-26 (2014)
3-acetylpyridine-induced degeneration in the adult ascidian neural complex: Reactive and regenerative changes in glia and blood cells.
Medina BN, et al.
Developmental neurobiology, 75, 877-877 (2015)
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