60128

Sigma-Aldrich

Potassium chloride

BioUltra, for molecular biology, ≥99.5% (AT)

Linear Formula:
KCl
CAS Number:
Molecular Weight:
74.55
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.26

Quality Level

grade

for molecular biology

product line

BioUltra

assay

≥99.5% (AT)

impurities

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected
≤0.001% total nitrogen (N)

loss

≤0.2% loss on drying, 110 °C

pH

5.0-8.0 (25 °C, 1 M in H2O)

mp

770 °C (lit.)

solubility

H2O: 1 M at 20 °C, clear, colorless

density

1.98 g/mL at 25 °C (lit.)

anion traces

bromide (Br-): ≤500 mg/kg
iodide (I-): ≤20 mg/kg
phosphate (PO43-): ≤5 mg/kg
sulfate (SO42-): ≤30 mg/kg

cation traces

Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤10 mg/kg
Bi: ≤5 mg/kg
Ca: ≤50 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤2 mg/kg
Li: ≤5 mg/kg
Mg: ≤10 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤200 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

λ

1 M in H2O

UV absorption

λ: 260 nm Amax: 0.01
λ: 280 nm Amax: 0.01

SMILES string

[Cl-].[K+]

InChI

1S/ClH.K/h1H;/q;+1/p-1

InChI key

WCUXLLCKKVVCTQ-UHFFFAOYSA-M

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Application

May be used for the preparation of phosphate buffered saline, and for the extraction and solubilization of proteins.

Other Notes

In DpnI/MboI assay for detection of methylation changes in DNA replication

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

M.L. DePamphilis
Methods in Enzymology, 262, 653-653 (1995)
Yuanyuan Qu et al.
Nucleic acids research, 41(10), 5263-5272 (2013-04-13)
Bacterial nucleoid-associated proteins, such as H-NS-like proteins in Enterobacteriaceae, are abundant DNA-binding proteins that function in chromosomal DNA organization and gene transcription regulation. The Mycobacterium tuberculosis Lsr2 protein has been proposed to be the first identified H-NS analogue in Gram-positive...
Linda K Månsson et al.
Faraday discussions, 181, 49-69 (2015-05-15)
We describe a route towards self-assembled colloidal molecules, where thermoresponsive microgels serve as discrete, externally tunable interaction sites. The ability of poly(N-isopropylacrylamide) (PNIPAM) and poly(N-isopropylmethacrylamide) (PNIPMAM) microgels to adsorb to the oil/water (O/W) interface and create Pickering-stabilized mini-emulsions was first...
Masatoshi Takagaki et al.
Experimental neurology, 252, 12-17 (2013-11-20)
Sedatives in the neurointensive care unit can strongly influence patients' risks of developing secondary brain damage. In particular, isoflurane, a volatile anesthetic, has been recently re-introduced to the neurointensive care unit, and first clinical studies suggest beneficial effects due to...
Qing-Fu Cao et al.
Molecular and cellular biology, 35(20), 3459-3470 (2015-07-29)
Restores TBP function 1 (Rtf1) is generally considered to be a subunit of the Paf1 complex (PAF1C), a multifunctional protein complex involved in histone modification and transcriptional or posttranscriptional regulation. Rtf1, however, is not stably associated with the PAF1C in...

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