MilliporeSigma
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48275

Sigma-Aldrich

β-Galactosidase from Escherichia coli

suitable for enzyme immunoassay, lyophilized, powder, ~140 U/mg

Synonym(s):
β-D-Galactoside galactohydrolase, Lactase
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
NACRES:
NA.54

form

powder

Quality Level

quality

lyophilized

specific activity

~140 U/mg

mol wt

Mr ~135000

contains

phosphate buffer as stabilizer
sucrose as stabilizer

technique(s)

enzyme immunoassay: suitable

shipped in

wet ice

storage temp.

−20°C

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Application

Aqueous solution may be used directly for derivatization without previous dialysis or gel permeation chromatography.

Biochem/physiol Actions

β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Physical properties

Tetramer molecular weight 465 kDa (subunits 116.3 kDa each)

Unit Definition

1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol 2-nitrophenyl-β-D-galactopyranoside (Cat. No. 73660) per minute at pH 7.8 and 37 °C

Other Notes

Used in a highly sensitive enzyme-linked immunoassay of human IgG with 4-methylumbelliferyl-β-D-galactoside (Catalog No. 69590)[77]; Evaluation of β-D-galactosidase (E. coli) and horse radish peroxidase as labels by sandwich enzyme immunoassay technique[78]; Enzyme immunoassay of thyrptropin releasing hormone (TRH)[79]; Preparation of enzyme-antibody conjugates[22]

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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