46393

Fluo-3

suitable for fluorescence, ~70%

• Synonym(s): 4-(2,7-Dichloro-6-hydroxy-3-oxo-9-xanthenyl)-4′-methyl-2,2′-(ethylenedioxy)dianiline-N,N,N′,N′-tetraacetic acid
• Empirical Formula (Hill Notation): C₃₆H₃₀Cl₂N₂O₁₃
• CAS Number: 123632-39-3
• Molecular Weight: 769.54
• MDL number: MFCD00083337
• PubChem Substance ID: 329757162
• NACRES: NA.32

Properties

• Quality Level: 100
• assay: ~70%
• form: solid
• solubility: H₂O: soluble
• fluorescence: λ_ex 506 nm; λ_em 526 nm in 0.1 M Tris pH 8.0, esterase 30 mM Ca²⁺
• suitability: suitable for fluorescence
• storage temp.: −20°C
• SMILES string: Cc1ccc(N(CC(O)=O)CC(O)=O)c(OCCOc2cc(ccc2N(CC(O)=O)CC(O)=O)C3=C4C=C(Cl)C(=O)C=C4Oc5cc(O)c(Cl)cc35)c1
• InChI: 1S/C36H30Cl2N2O13/c1-18-2-4-24(39(14-32(43)44)15-33(45)46)30(8-18)51-6-7-52-31-9-19(3-5-25(31)40(16-34(47)48)17-35(49)50)36-20-10-22(37)26(41)12-28(20)53-29-13-27(42)23(38)11-21(29)36/h2-5,8-13,41H,6-7,14-17H2,1H3,(H,43,44)(H,45,46)(H,47,48)(H,49,50)
• InChI key: OZLGRUXZXMRXGP-UHFFFAOYSA-N

Description

Application

Fluo-3 and related molecule Fluo3/AM are used as a fluorescence indicator of intracellular calcium (Ca2+). Fluo-3 may be use for flow cytometry and confocal laser scanning microscopy using visible light excitation (compatible with argon laser sources operating at 488 nm). Fluorescence intensity increases about 40-fold after calcium binding.

Other Notes

Fluorescent indicator for calcium used in flow cytometry. Fluorescence intensity increases about 40-fold after calcium binding

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves