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TR146 cell line human

Quality Level

biological source

human mouth


Human squamous cell carcinoma

growth mode



Not specified




Not specified


Not specified


cell culture | mammalian: suitable

relevant disease(s)


shipped in

dry ice

storage temp.


Related Categories

General description

The TR146 cells are appropriate for drug transport studies and they mimic normal human buccal epithelium. It serves as an oral mucosa model for barrier property studies with various molecules including tight junction proteins, aquaporins and epithelial-mesenchymal transition (EMT) markers.

Cell Line Origin

Human squamous cell carcinoma

Cell Line Description

TR146 was derived from the neck node of a 67 year-old female (primary tumour was sited in buccal mucosa). The patient had had previous radiotherapy (6,000 rads) and neck dissection. TR146 is tumourigenic in female (nu/nu) mice leading to the production of rapidly progressing tumours. Histology: well-differentiated keratinizing squamous cell carcinoma.


TR146 cell line human has been used:
  • in cytotoxicity studies with an antibacterial compound, metronidazole
  • in viability and metabolic studies post blue laser light exposure,
  • as an oral epithelium-resembling cell line model for in vitro C. albicans infection studies
  • in barrier function studies using transepithelial electrical resistance (TEER)
  • for the transport studies with C-reactive protein (CRP)

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 11,13
D13S317: 11,14
D16S539: 13
D5S818: 10,11
D7S820: 10,12
THO1: 9
TPOX: 8,9
vWA: 15,18

Culture Medium

HAMS F12 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

Subculture Routine

Split sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at approximately 3 x 104 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Saturation density of 5 x 104 cells/cm2.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.


This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.

Storage Class Code

12 - Non Combustible Liquids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

J Jacobsen et al.
European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V, 48(3), 217-224 (1999-12-28)
The dissolution rate, the toxicity and the release from chewing gum of miconazole and econazole cyclodextrin products and complexes were investigated. The dissolution rate studies showed that an amorphous miconazole hydroxypropyl-beta-cyclodextrin product gave drug supersaturation, whereas drug supersaturation was not
M Pedersen et al.
Drug development and industrial pharmacy, 25(4), 463-470 (1999-04-09)
Parameters that influence the precipitation of the beta-cyclodextrin (beta-CD) inclusion complexes of the antimycotics miconazole and econazole were investigated. The mechanistic reason for the superior antimycotic activity of the miconazole inclusion complex was studied. The toxicity of the complex was
Tissue barriers, 8(2), 1784644-1784644 (2020-07-03)
H T Rupniak et al.
Journal of the National Cancer Institute, 75(4), 621-635 (1985-10-01)
Four human cell lines were established from biopsy specimens of squamous cell carcinomas of the larynx (TR131 and TR138), tongue (TR126), and buccal mucosa that had infiltrated a lymph node (TR146). All 4 lines readily formed colonies on a plastic
Hanne Mørck Nielsen et al.
European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences, 16(3), 151-157 (2002-07-20)
The present study was conducted to investigate and compare the effect of pH and drug concentration on nicotine permeability across the TR146 cell culture model and porcine buccal mucosa in vitro. As a further characterization of the TR146 cell culture

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