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TR146 cell line human

Synonym(s):
TR-146 Cells

Quality Level

biological source

human mouth

description

Human squamous cell carcinoma

growth mode

Adherent

karyotype

Not specified

morphology

Polygonal

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

General description

The TR146 cells are appropriate for drug transport studies and they mimic normal human buccal epithelium. It serves as an oral mucosa model for barrier property studies with various molecules including tight junction proteins, aquaporins and epithelial-mesenchymal transition (EMT) markers.

Cell Line Origin

Human squamous cell carcinoma

Cell Line Description

TR146 was derived from the neck node of a 67 year-old female (primary tumour was sited in buccal mucosa). The patient had had previous radiotherapy (6,000 rads) and neck dissection. TR146 is tumourigenic in female (nu/nu) mice leading to the production of rapidly progressing tumours. Histology: well-differentiated keratinizing squamous cell carcinoma.

Application

TR146 cell line human has been used:
  • in cytotoxicity studies with an antibacterial compound, metronidazole
  • in viability and metabolic studies post blue laser light exposure,
  • as an oral epithelium-resembling cell line model for in vitro C. albicans infection studies
  • in barrier function studies using transepithelial electrical resistance (TEER)
  • for the transport studies with C-reactive protein (CRP)

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 11,13
D13S317: 11,14
D16S539: 13
D5S818: 10,11
D7S820: 10,12
THO1: 9
TPOX: 8,9
vWA: 15,18

Culture Medium

HAMS F12 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

Subculture Routine

Split sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at approximately 3 x 104 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Saturation density of 5 x 104 cells/cm2.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Disclaimer

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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