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X114

Sigma-Aldrich

Triton X-114

laboratory grade

Synonym(s):
(1,1,3,3-Tetramethylbutyl)phenyl-polyethylene glycol, Polyethylene glycol tert-octylphenyl ether
Linear Formula:
(C2H4O)n C14H22O, n = 7 or 8
CAS Number:
Beilstein:
2315025
MDL number:
PubChem Substance ID:
NACRES:
NA.21

Quality Level

grade

laboratory grade

description

non-ionic

mol wt

average mol wt 537

composition

t-Oct-C6H4-(OCH2CH2)xOH, x = 7-8

refractive index

n20/D 1.488 (lit.)

CMC

0.2 mM (20-25°C)

transition temp

pour point ~−9 °C
cloud point 23 °C

density

1.058 g/mL at 25 °C (lit.)

HLB

12.4

SMILES string

CC(C)(C)CC(C)(C)c1ccc(OCCOCCOCCOCCOCCOCCOCCO)cc1

InChI

1S/C28H50O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h6-9,29H,10-24H2,1-5H3

InChI key

HNLXNOZHXNSSPN-UHFFFAOYSA-N

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General description

Triton X-114 is a nonionic detergent. It is a good detergent for solubilizing membranes having reversible phase-separation property useful for the separation of intrinsic membrane proteins. Solution of Triton X-114 is homogeneous at 0°C but phase separation into aqueous phase and a detergent phase occurs above 20°C. Phase separation of proteins in solutions of Triton X-114 has been investigated. This octylphenol ethoxylate is compatible with anionic, cationic, and other nonionic surfactants and chemically stable in most acidic & alkaline solutions.

Application

Triton X-114 has been used:
  • To remove endotoxin from solutions of protein using phase separation technique
  • In the extraction and purification of DNA from endotoxin
  • In the biphasic extraction of lipoproteins from bacterial cell wall
  • As a non-ionic detergent with a low cloud point (23 °C) enabling protein solubilization with phase-partitioning of hydrophilic from amphiphilic proteins

Packaging

1 gal in poly bottle
5 gal in poly drum
1 L in poly bottle
100, 500 mL in poly bottle

Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Signal Word

Danger

Hazard Statements

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

483.8 °F

Flash Point(C)

251 °C

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

Mechanism and function of a newly identified CpG DNA motif in human primary B cells.
Hartmann G and Krieg A M
Journal of Immunology, 164(2), 944-953 (2000)
Huanhuan Li et al.
Life sciences, 231, 116548-116548 (2019-06-11)
Multidrug resistance is a major obstacle in chemotherapy, which is mainly caused by the overexpression of ATP-binding cassette (ABC) transporters. Breast cancer resistance protein (BCRP) is one of the ABC transporters and is strongly associated with multidrug resistance. Results of...
G Gabarrini et al.
Journal of dental research, 97(5), 556-562 (2018-01-05)
Porphyromonas gingivalis is one of the major oral pathogens implicated in the widespread inflammatory disorder periodontitis. Moreover, in recent years, P. gingivalis has been associated with the autoimmune disease rheumatoid arthritis. The peptidylarginine deiminase enzyme of P. gingivalis (PPAD) is...
TLR2 and its co-receptors determine responses of macrophages and dendritic cells to lipoproteins of Mycobacterium tuberculosis.
Golenbock D T, et al.
Cellular Immunology, 258(1), 29-37 (2009)
Mycobacterium tuberculosis LprG (Rv1411c): a novel TLR-2 ligand that inhibits human macrophage class II MHC antigen processing.
Gehring A J, et al.
Journal of Immunology, 173(4), 2660-2668 (2004)

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