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Tryptic Soy Broth

Microbiologically tested., NutriSelect® Basic

Soybean Casein digest Broth, Casein Soya Broth, CASO Broth, TSB, Tryptone Soya Broth, Soybean Casein digest Broth, Casein Soya Broth, CASO Broth, TSB
MDL number:

Quality Level






Microbiologically tested.


poly bottle of 1 kg
poly bottle of 500 g


NutriSelect® Basic


food and beverages
pathogen testing



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General description

Tryptic Soy Broth is a general-purpose liquid medium used in qualitative procedures for the sterility testing, enrichment, and cultivation of fastidious microorganisms. The formulation contains a combination of casein and soy peptones supplying organic nitrogen, particularly amino acids and long-chained peptides. Sodium chloride maintains the osmotic equilibrium and dextrose serves as a source of carbohydrate and energy.


Tryptic Soy Broth is a widely used medium for culturing aerobic gram-positive, gram-negative bacteria, fungus, routine blood cultures, and microbial biofilms. It is also been used as a growth medium to culture Staphylococcus aureus strains and as a growth medium for E.coli in the bacterial-killing assay (BKA) with bird plasma samples.


500 g in poly bottle
1 kg in poly bottle


Casein (pancreatic digest) 17 g/L
Soya peptone (papaic digest) 3 g/L
Sodium chloride 5 g/L
Dipotassium phosphate 2.5 g/L
Dextrose 2.5 g/L

Preparation Note

Suspend 30 g of dehydrated media in 1 litre of purified filtered water. Heat with frequent agitation and boil for one minute. Sterilize at 121°C for 15 minutes. Cool to 45-50°C. Mix gently and dispense into sterile Petri dishes or sterile culture tubes.


We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Sisi Gao et al.
The Journal of experimental biology, 220(Pt 2), 322-327 (2016-11-05)
Stress-induced effects on innate immune activity in wild birds have been difficult to predict. These difficulties may arise from the frequent assumptions that (1) the stress response influences different components of the immune response similarly, (2) stress-induced effects do not
Susannah S French et al.
Biology open, 1(5), 482-487 (2012-12-06)
The field of ecoimmunology is currently undergoing rapid expansion, whereby biologists from a wide range of ecological disciplines are increasingly interested in assessing immunocompetence in their study organisms. One of the key challenges to researchers is determining what eco-immune measures
Pavlos Anastasiadis et al.
Journal of nanobiotechnology, 12, 24-24 (2014-07-07)
Immuno-compromised patients such as those undergoing cancer chemotherapy are susceptible to bacterial infections leading to biofilm matrix formation. This surrounding biofilm matrix acts as a diffusion barrier that binds up antibiotics and antibodies, promoting resistance to treatment. Developing non-invasive imaging
Rouba Ghobeira et al.
Biomedical materials (Bristol, England), 12(1), 015017-015017 (2017-01-25)
For most tissue engineering applications, surface modification and sterilization of polymers are critical aspects determining the implant success. The first part of this study is thus dedicated to modifying polycaprolactone (PCL) surfaces via plasma treatment using a medium pressure dielectric
Ping Zhang et al.
The Journal of antimicrobial chemotherapy, 73(3), 615-619 (2017-12-07)
Staphylococcus aureus small colony variants (SCVs) cause persistent infections and are resistant to cationic antibiotics. Antimicrobial peptides (AMPs) have been suggested as promising alternatives for treating antibiotic-resistant bacteria. We investigated the capacity of the human cationic AMP LL-37 to kill

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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