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Pharmaceutical Secondary Standard; Certified Reference Material

Methylidyne trichloride, Trichloromethane, Chloroform
Empirical Formula (Hill Notation):
CAS Number:
Molecular Weight:
EC Number:
MDL number:
PubChem Substance ID:

Quality Level


certified reference material
pharmaceutical secondary standard

vapor density

4.1 (vs air)

vapor pressure

160 mmHg ( 20 °C)


current certificate can be downloaded


ampule of 3x1.2 mL each


HPLC: suitable
gas chromatography (GC): suitable

refractive index

n20/D 1.445 (lit.)


60.5-61.5 °C (lit.)


−63 °C (lit.)


1.492 g/mL at 25 °C (lit.)


pharmaceutical (small molecule)



pharmacopeia traceability

traceable to USP 1601383

storage temp.


SMILES string




InChI key


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General description

Chloroform is a volatile chlorinated organic solvent whose vapors have a narcotic effect. It is a widely used industrial and laboratory solvent. It is also used in the manufacture of many pesticides, rubber, resin, and wax.
Pharmaceutical secondary standards for application in quality control, provide pharma laboratories and manufacturers with a convenient and cost-effective alternative to the preparation of in-house working standards.


These Secondary Standards are qualified as Certified Reference Materials. These are suitable for use in several analytical applications including but not limited to pharma release testing, pharma method development for qualitative and quantitative analyses, food and beverage quality control testing, and other calibration requirements.
Chloroform may be used as a solvent reference standard for the determination of the analyte in bulk drug and dosage forms by spectrophotometry and spectrofluorimetry techniques.

Analysis Note

These secondary standards offer multi-traceability to the USP, EP and BP primary standards, where they are available.

Other Notes

This Certified Reference Material (CRM) is produced and certified in accordance with ISO 17034 and ISO/IEC 17025. All information regarding the use of this CRM can be found on the certificate of analysis.


To see an example of a Certificate of Analysis for this material enter LRAA8822 in the slot below. This is an example certificate only and may not be the lot that you receive.


Skull and crossbonesHealth hazard

Signal Word


Hazard Classifications

Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 - STOT SE 3

Target Organs

Central nervous system

Storage Class Code

6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects



Flash Point(F)

does not flash

Flash Point(C)

does not flash

Certificate of Analysis

Certificate of Origin

Spectrophotometric and spectrofluorimetric determination of some drugs containing secondary amino group in bulk drug and dosage forms via derivatization with 7-chloro-4-nitrobenzofurazon
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