MIC007

Supelco

E. coli in Drinking and Surface Water PT- Quantitative - WS

Proficiency Testing Material

NACRES:
NA.85

Quality Level

packaging

set of 2 ea

matrix

Vitroid

Featured Industry

Environmental
Food and Beverages

shipped in

wet ice

storage temp.

−20°C

suitability

Escherichia coli
coliforms

General description

E. coli in Drinking and Surface Water PT - Quantitative is produced in accordance with ISO/IEC 17043:2010. This sample is manufactured according to TNI requirements. However, please note, this sample may include non - TNI analytes as well. The PT portfolio allows our test components to offer significant performance data to monitor and prove competency to customers, accreditation bodies or both.

Supelco® is a global proficiency testing (PT) provider that manufactures best-in-class environmental and pharmaceutical PT products. Most water PT samples are available as concentrates or ready-to-use volumes giving the end-user flexibility in selecting samples that will fit their exact needs.
These samples are typically provided in duplicate for use in case of lab mishap, for QC purposes or to analyze multiple methods.

Type of PT Scheme: Water analysis - Water Supply (WS)

Matrix: Water Supply/Drinking water

Analyte/s: Values of analytes vary lot to lot.

All information regarding the use of E. coli in Drinking and Surface Water PT - Quantitative proficiency testing standard can be found in the reporting packet which will be available to download at PT Portal,.

Application

E. coli in Drinking and Surface Water PT - Quantitative proficiency testing material is designed for the quantitative determination of E. coli in drinking water samples.

Have any questions? Explore our FAQs,.
For more information on this product, please contact our PT Service Team,.

Features and Benefits

We have a vast portfolio of environmental PT schemes with "fit-for-purpose" samples to help you meet the needs of the laboratory.

Our proficiency testing (PT) program is a benchmark to:

  • provide staff with an insight into their laboratory′s performance, to verify the accuracy and reliability of their testing process
  • provide a comparison of performance with that of other laboratories, nationally and internationally
  • test and educate staff, provide a better understanding of the impact of incorrect results and identify areas where there may be problems
  • provide an independent evaluation of laboratory performance
  • demonstrate to clients, colleagues and accreditation bodies that there is a commitment to quality

Packaging

Set contains 2 sterile screw cap vials each containing a Vitroid (a small disk containing the possible organisms) and 2 - 99mL bottles of phosphate buffer.
(Please note, buffer solution may not be provided with your sample. If this causes any concern, please contact PT Service ptservice@milliporesigma.com.)

Preparation Note

Description

  • This PT sample is designed for the quantitative analysis of total and/or fecal coliform.
  • The PT sample is provided in duplicate as a stable dehydrated Vitroid requiring hydration. The additional sample is provided for mishaps in the lab. TNI prohibits the practice of multiple tests on a PT sample unless it is normal laboratory practice and multiple analysis is conducted on normal day to day samples.
  • A 100 mL of sterile hydration fluid is supplied with the PT sample.(Please note, buffer solution may not be provided with your sample. If this causes any concern, please contact PT Service ptservice@milliporesigma.com.) This is a phosphate buffer. If you are following IDEXX methods for Quanti-Tray® do not use the buffer provided and follow the directions provided with the media (suggests using Type 1 DI water.)
  • The dehydrated Vitroid sample is stable for a time exceeding the study schedule.

General Information

  • Due to the sensitivity of microorganisms and short holding times after hydration, please read the entire instructions prior to preparation.
  • This sample is a single Vitroid and the possible concentration range is quite broad, it may be necessary to analyze the sample in a dilution series using general phosphate buffer solution.
  • This sample may be analyzed by any method appropriate for coliforms.
  • The hydration temperature of 35 - 37 °C is rigorous. Low temperature will not assure complete hydration. Higher temperatures may kill the microorganisms.

Preparation Instructions

  • Allow the capped sample vial to equilibrate to room temperature.
  • While the sample is equilibrating to room temperature, warm the hydrating fluid in your incubator to 35 - 37 °C. This will take about 60 min.
  • The hydrating fluid is warmed to 35 - 37 °C, open the sample vial and aseptically transfer the Vitroid to the hydrating fluid. NOTE: The Vitroid is the colored disk within the cryotube. It will either be on top of cotton with opaque desiccant beads below the cotton or free moving in a cryotube that contains a desiccant tablet within the cap. The opaque desiccant beads and desiccant tablet are to keep the container dry and are not biologicals.
  • Shake well and place the sample in your incubator for 15 min.
  • The hydration temperature of 35 - 37 °C is rigorous. Low temperature will not assure complete hydration. Higher temperatures may kill the microorganisms.
  • After 15 min, remove the hydrated sample from the incubator and shake well. Inspect to assure dissolution of the Vitroid. The disk will dissolve completely.
  • This represents the sample for analysis. Further dilutions are recommended to achieve a proper count, but base all counts on the whole volume sample.

Storage and Stability

Please refer to sample label for expiration date, specific storage temperature, and specific storage conditions.

Other Notes

Are you new to Supelco® Proficiency Testing? Be sure to register your account details, in the PT Portal before placing your order.
This will provide access to data entry, reporting packets and final reports. Registrations will be approved shortly after your order is placed. For further questions, write to us at ptservice@milliporesigma.com.

When placing your order online, please indicate your study preference under the ′your reference′ column. Available studies can be viewed at the bottom of the page.
Click here, to learn more about Quick-Turn Studies (on-demand proficiency tests that are offered depending on lot availability). The Quick-Turn results are released within 48-72 hours whereas scheduled study reports are released within 21 days.

Proficiency testing samples are presented as single blind. No values for the samples are released until the testing period concludes.

This PT sample is designed for the quantitative analysis of total and/or fecal coliform. The PT sample is provided in duplicate as a stable dehydrated vitroid requiring hydration. The additional sample is provided for mishaps in the lab. Store the PT sample at as directed. The dehydrated vitroid sample is stable for a time exceeding the study schedule.

Proficiency Testing Schedule (2021-2022)

Drinking Water
WS21-1January 13 - February 26, 2021
WS21-2March 24 - May 7, 2021
WS21-3 July 7 - August 20, 2021
WS21-4 September 22 - November 5, 2021

WS22-1January 12 - February 25, 2022
WS22-2March 23 - May 6, 2022
WS22-3 July 6 - August 19, 2022
WS22-4 September 21 - November 4, 2022

Legal Information

Quanti-Tray is a registered trademark of IDEXX Laboratories, Inc.
Supelco is a registered trademark of Merck KGaA, Darmstadt, Germany
Vitroid is a trademark of Sigma-Aldrich Co. LLC

Analyte

Description

    Total coliforms 20.0 - 200 CFU/100mL
    Total Coliform, MPN 20.0 - 200 MPN/100 mL
    Escherichia coli 20.0 - 200 CFU/100mL
    Escherichia coli, MPN 20.0 - 200 MPN/100 mL
    Fecal coliforms 20.0 - 200 CFU/100mL
    Fecal coliform, MPN 20.0 - 200 MPN/100 mL

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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