A3902

Bovine Serum Albumin

lyophilized powder, Vitamin B₁₂ deficient

• Synonym(s): Albumin bovine serum, Bovine albumin, BSA
• CAS Number: 9048-46-8
• EC Number: 232-936-2
• MDL number: MFCD00130384

Properties

• biological source: bovine
• description: ≤ 1.0ug/g vitamin B₁₂ content
• assay: ≥98%
• form: lyophilized powder
• mol wt: ~66 kDa
• purified by: heat shock fractionation
• origin: USA origin
• technique(s): ELISA: suitable
• loss: ≤5%
• pH: 7
• solubility: water: soluble (40 mg/ml)
• suitability: suitable for (Suitable for use in vitamin B12 assays.)
• UniProt accession no.: P02769
• storage temp.: 2-8°C
• Gene Information: bovine ... ALB(280717)

Description

Application

For use in vitamin B₁₂ assays.

Biochem/physiol Actions

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Preparation Note

Prepared using heat shock fractionation

Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.