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Millipore

m-Endo Broth

NutriSelect® Plus, suitable for microbiology

Synonym(s):

Membrane Endo Broth

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About This Item

UNSPSC Code:
41171606
NACRES:
NA.85

form

powder

Quality Level

200

shelf life

limited shelf life, expiry date on the label

composition

basic fuchsin, 1 g/L
dipotassium phosphate, 7 g/L
lactose, 25 g/L
peptic digest of animal tissue, 20 g/L
sodium sulfite, 2.5 g/L
yeast extract, 6 g/L

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

final pH

7.5±0.2 (25 °C)

application(s)

agriculture
environmental
food and beverages
water monitoring
microbiology

suitability

selective for Escherichia coli
selective for coliforms

Application

m-Endo Broth is used for the estimation of coliforms in water samples using membrane filter technique.

Preparation Note

Suspend 61.5 g in 1 litre distilled water. Heat to dissolve the medium completely. Sterilize by autoclaving at 121°C for 5 minutes. The medium should be used on the same day of its rehydration.
Caution: Basic fuchsin is a potential carcinogen and care should be taken to avoid inhalation of the powdered dye and contamination of the skin.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Health hazard
GHS08

signalword

Warning

hcodes

H351

Hazard Classifications

Carc. 2

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
BCCD4880
BCCB4466
BCBX8504
BCBV0544
BCBS5129V

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Electron microscopic investigation of the bactericidal action of electrochemical disinfection in comparison with chlorination, ozonation and Fenton reaction.
Diao HF, et al.
Process Biochemistry (Oxford, United Kingdom), 39(11), 1421-1426 (2004)
J D Berg et al.
Applied and environmental microbiology, 54(8), 2118-2122 (1988-08-01)
Three fluorogenic methylumbelliferone (MU) substrates were evaluated for rapid detection of total and fecal coliform bacteria (TC and FC) in drinking water. 4-MU-beta-D-galactoside, MU-heptanoate, and MU-glucuronide were used to determine enzyme activity as a surrogate measure of coliform concentration. Coliforms

Articles

Selective Growth Media for Differentiation and Detection of Escherichia coli and Other Coliforms

An article concerning selective growth media for differentiation and detection of Escherichia coli and other coliforms.

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