11814524001

rGFP

Green Fluorescent Protein from jellyfish Aequorea victoria, recombinant expressed in E. coli

• Synonym(s): gfp, r-, green fluorescent protein

Properties

• recombinant: expressed in E. coli
• Quality Level: 100
• form: solution
• packaging: pkg of 50 μL (1 mg/ml)
• manufacturer/tradename: Roche
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

GFP isolated from jellyfish Aequorea Victoria exhibited spontaneous fluorescence property. It is a novel important marker for gene expression in various heterologous systems. It is stably present in plant cells and shows photobleaching. Molecular cloning of GFP produces a fused protein with fluorescence excitation and emission spectra.

Specificity

Heat inactivation: Fluorescence will be quenched by reducing reagents, such as DTT or sodiumdithiosulfate, and is completely inhibited by peroxide.
Fluorescence is inhibited by extreme pH conditions (pH < 4 and pH > 11) as well as ethanol content higher than 60%.

Application

Recombinant Green Fluorescent Protein (rGFP) is suitable as:

• Positive control when detecting GFP fusion proteins by SDS-PAGE.
• Positive control when detecting GFP fusion proteins by western blot using Anti-GFP monoclonal antibody.
• Standard for experiments involving fluorescence microscopy.

Recombinant green fluorescent protein (rGFP) has also been used in indirect enzyme-linked immunosorbent assay.

Physical form

Solution, 1 mg/ml rGFP in 5 mM Tris-HCl, 5 mM EDTA, pH 8.0

Preparation Note

Working concentration: Amount of rGFP recommended for:

• SDS-PAGE (Coomassie stained): 1 μg
• Western blot: 5 ng

Other Notes

Emission max: 509nm
Excitation max: 395nm

For life science research only. Not for use in diagnostic procedures.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(C): No data available