MilliporeSigma
All Photos(1)

11814524001

Roche

rGFP

Green Fluorescent Protein from jellyfish Aequorea victoria, recombinant expressed in E. coli

Synonym(s):
gfp, r-, green fluorescent protein

recombinant

expressed in E. coli

Quality Level

form

solution

packaging

pkg of 50 μL (1 mg/ml)

manufacturer/tradename

Roche

shipped in

wet ice

storage temp.

2-8°C

General description

GFP isolated from jellyfish Aequorea Victoria exhibited spontaneous fluorescence property. It is a novel important marker for gene expression in various heterologous systems. It is stably present in plant cells and shows photobleaching. Molecular cloning of GFP produces a fused protein with fluorescence excitation and emission spectra.

Specificity

Heat inactivation: Fluorescence will be quenched by reducing reagents, such as DTT or sodiumdithiosulfate, and is completely inhibited by peroxide.
Fluorescence is inhibited by extreme pH conditions (pH < 4 and pH > 11) as well as ethanol content higher than 60%.

Application

Recombinant Green Fluorescent Protein (rGFP) is suitable as:
  • Positive control when detecting GFP fusion proteins by SDS-PAGE.
  • Positive control when detecting GFP fusion proteins by western blot using Anti-GFP monoclonal antibody.
  • Standard for experiments involving fluorescence microscopy.
Recombinant green fluorescent protein (rGFP) has also been used in indirect enzyme-linked immunosorbent assay.

Physical form

Solution, 1 mg/ml rGFP in 5 mM Tris-HCl, 5 mM EDTA, pH 8.0

Preparation Note

Working concentration: Amount of rGFP recommended for:
  • SDS-PAGE (Coomassie stained): 1 μg
  • Western blot: 5 ng

Other Notes

Emission max: 509nm
Excitation max: 395nm
For life science research only. Not for use in diagnostic procedures.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(C)

No data available

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

SDS

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service