11814524001

Roche

rGFP

Green Fluorescent Protein from jellyfish Aequorea victoria, recombinant expressed in E. coli

Synonym(s):
green fluorescent protein, gfp, r-

Quality Level

form

solution

packaging

pkg of 50 μL (1 mg/ml)

manufacturer/tradename

Roche

shipped in

wet ice

storage temp.

2-8°C

General description

GFP isolated from jellyfish Aequorea Victoria exhibited spontaneous fluorescence property. It is a novel important marker for gene expression in various heterologous systems. It is stably present in plant cells and shows photobleaching. Molecular cloning of GFP produces a fused protein with fluorescence excitation and emission spectra.

Specificity

Heat inactivation: Fluorescence will be quenched by reducing reagents, such as DTT or sodiumdithiosulfate, and is completely inhibited by peroxide.
Fluorescence is inhibited by extreme pH conditions (pH < 4 and pH > 11) as well as ethanol content higher than 60%.

Application

Recombinant green fluorescent protein (rGFP) has also been used in indirect enzyme-linked immunosorbent assay.
Recombinant Green Fluorescent Protein (rGFP) is suitable as:
  • Positive control when detecting GFP fusion proteins by SDS-PAGE.
  • Positive control when detecting GFP fusion proteins by western blot using Anti-GFP monoclonal antibody.
  • Standard for experiments involving fluorescence microscopy.

Physical form

Solution, 1 mg/ml rGFP in 5 mM Tris-HCl, 5 mM EDTA, pH 8.0

Preparation Note

Working concentration: Amount of rGFP recommended for:
  • SDS-PAGE (Coomassie stained): 1 μg
  • Western blot: 5 ng

Other Notes

Emission max: 509nm
Excitation max: 395nm
For life science research only. Not for use in diagnostic procedures.

storage_class_code

12 - Non Combustible Liquids

WGK Germany

WGK 1

Flash Point(F)

No data available

Flash Point(C)

No data available

Certificate of Analysis

Certificate of Origin

S Inouye et al.
FEBS letters, 341(2-3), 277-280 (1994-03-21)
Expression of the cDNA for Aequorea green fluorescent protein in E. coli yielded a fused protein with fluorescence excitation and emission spectra virtually identical to those of the native green fluorescent protein. Further, a solution of the protein, when mixed...
Methods for addressing the protein-protein interaction between histone deacetylase 6 and ubiquitin
dos Santos Passos C, et al.
bioRxiv, 203372-203372 (2017)
J Sheen et al.
The Plant journal : for cell and molecular biology, 8(5), 777-784 (1995-11-01)
The green-fluorescent protein (GFP) from jellyfish Aequorea victoria has been used as a convenient new vital marker in various heterologous systems. However, it has been problematic to express GFP in higher eukaryotes, especially in higher plants. This paper reports that...
Efficient central nervous system AAVrh10-mediated intrathecal gene transfer in adult and neonate rats.
Hordeaux J, et al.
Gene Therapy, 22(4), 316-316 (2015)
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