11814150001

Anti-c-myc-Peroxidase

from mouse IgG₁κ;

• Synonym(s): anti-myc-peroxidase

Properties

• biological source: mouse
• Quality Level: 100
• conjugate: peroxidase conjugate
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 9E10, monoclonal
• Assay: 90% (HPLC and SDS-PAGE)
• form: solution
• packaging: pkg of 500 μg (500 μl)
• manufacturer/tradename: Roche
• isotype: IgG1κ
• epitope sequence: EQKLISEEDL
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

Anti-c-myc-Peroxidase is a monoclonal antibody to c-myc peptide (clone 9E10), conjugated to horseradish peroxidase. Anti-c-myc recognizes the 9E10 epitope (EQKLISEEDL), derived from the human c-myc protein. The monoclonal antibody against the c-myc epitope is well characterized and does not cross-react with other cellular proteins. The antibody recognizes its antigenic determinant even when the c-myc-peptide epitope is introduced into unrelated recombinant proteins by a technique known as "epitope tagging".

Specificity

Anti-c-myc-Peroxidase recognizes the 9E10 epitope (EQKLISEEDL), which was derived from the human c-myc protein. The monoclonal antibody against the c-myc epitope is well characterized and does not crossreact with other cellular proteins. The antibody recognizes its antigenic determinant even when the c-myc peptide epitope is introduced into unrelated recombinant proteins either N-terminal, C-terminal or internal.

Immunogen

Amino acid sequence EQKLISEEDL was derived from the human c-myc protein.

Application

• Use Anti-c-myc-Peroxidase for the detection of native human c-myc protein and recombinant epitope-tagged proteins that contain the c-myc epitope using: Dot blots
• Western blots

Quality

Function test: Western blot using a cell line that expresses a recombinant c-myc-tagged protein.

Preparation Note

Working concentration: Working concentration of conjugate depends on application and substrate.
The following concentrations should be taken as a guideline:

• Western blot: 1 μg/ml

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(C): No data available