MilliporeSigma
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11814150001

Roche

Anti-c-myc-Peroxidase

from mouse IgG1κ;

Synonym(s):
anti-myc-peroxidase

biological source

mouse

Quality Level

conjugate

peroxidase conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

9E10, monoclonal

assay

90% (HPLC and SDS-PAGE)

form

solution

packaging

pkg of 500 μg (500 μl)

manufacturer/tradename

Roche

isotype

IgG1κ

epitope sequence

EQKLISEEDL

shipped in

dry ice

storage temp.

−20°C

General description

Anti-c-myc-Peroxidase is a monoclonal antibody to c-myc peptide (clone 9E10), conjugated to horseradish peroxidase. Anti-c-myc recognizes the 9E10 epitope (EQKLISEEDL), derived from the human c-myc protein. The monoclonal antibody against the c-myc epitope is well characterized and does not cross-react with other cellular proteins. The antibody recognizes its antigenic determinant even when the c-myc-peptide epitope is introduced into unrelated recombinant proteins by a technique known as "epitope tagging".

Specificity

Anti-c-myc-Peroxidase recognizes the 9E10 epitope (EQKLISEEDL), which was derived from the human c-myc protein. The monoclonal antibody against the c-myc epitope is well characterized and does not crossreact with other cellular proteins. The antibody recognizes its antigenic determinant even when the c-myc peptide epitope is introduced into unrelated recombinant proteins either N-terminal, C-terminal or internal.

Immunogen

Amino acid sequence EQKLISEEDL was derived from the human c-myc protein.

Application

  • Use Anti-c-myc-Peroxidase for the detection of native human c-myc protein and recombinant epitope-tagged proteins that contain the c-myc epitope using: Dot blots
  • Western blots

Quality

Function test: Western blot using a cell line that expresses a recombinant c-myc-tagged protein.

Preparation Note

Working concentration: Working concentration of conjugate depends on application and substrate.
The following concentrations should be taken as a guideline:
  • Western blot: 1 μg/ml

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

No data available

Flash Point(C)

No data available

Certificate of Analysis

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Certificate of Origin

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SDS

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