Especially purified for membrane research
Triton X-100 is used:
- as one of the most commonly used non-ionic aqueous detergents for solubilizing membrane proteins during isolation of membrane-protein complexes
- for native gel electrophoresis
- as a blocking agent in immunocytochemistry
- for permeabilization of slides prior to immunostaining and immunofluorescence
- for permeabilization of slides used for TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling) technique
Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.
Aqueous solution, 10%(w/v), filled under nitrogen atmosphere in injection vials
Purity: <2 ppm peroxide content (as H2O2),<200 μM carbonyls,<100 μS conductivity
Note: Purified to reduce levels of unwanted peroxides, carbonyls, metals, and salts. To preserve the low peroxide content of the preparation, always remove aliquots of detergent from the vial with a syringe.
C34H62O11, for n = 10
Working concentration: 1-5 mM
CMC**: approximately 0.2 mM at +25°C
** CMC: critical micellar concentration
For life science research only. Not for use in diagnostic procedures.
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow