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11332481001

Sigma-Aldrich

Triton X-100

pkg of 5 × 10 mL, suitable for protein purification, suitable for DNA extraction, suitable for cell culture

Synonym(s):
4-(1,1,3,3-Tetramethylbutyl)phenyl-polyethylene glycol solution, t-Octylphenoxypolyethoxyethanol, Polyethylene glycol tert-octylphenyl ether
Linear Formula:
t-Oct-C6H4-(OCH2CH2)xOH, x= 9-10
CAS Number:
Beilstein:
2315025
MDL number:
PubChem Substance ID:

form

solution

mol wt

micellar avg mol wt 80,000
Mr = 647 (for n=10)
average mol wt 625

packaging

pkg of 5 × 10 mL

manufacturer/tradename

Roche

aggregation number

100-155

technique(s)

DNA extraction: suitable
cell culture | mammalian: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
protein purification: suitable

CMC

0.2-0.9 mM (20-25°C)

transition temp

cloud point 65 °C
pour point ~7 °C

absorption

= 0.88 at 254 nm at 0.2 % (w/v)
= 1.11 at 278 nm at 0.5 % (w/v)

HLB

13.5

shipped in

wet ice

storage temp.

2-8°C

SMILES string

CC(C)(C)CC(C)(C)c1ccc(OCCOCCOCCOCCOCCOCCOCCO)cc1

InChI

1S/C28H50O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h6-9,29H,10-24H2,1-5H3

InChI key

HNLXNOZHXNSSPN-UHFFFAOYSA-N

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General description

Octophenolpoly(ethyleneglycolether)n
Especially purified for membrane research

Application

Triton X-100 is used:
  • as one of the most commonly used non-ionic aqueous detergents for solubilizing membrane proteins during isolation of membrane-protein complexes
  • for native gel electrophoresis
  • as a blocking agent in immunocytochemistry
  • for permeabilization of slides prior to immunostaining and immunofluorescence
  • for permeabilization of slides used for TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling) technique

Biochem/physiol Actions

Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.

Components

Aqueous solution, 10%(w/v), filled under nitrogen atmosphere in injection vials

Quality

Purity: <2 ppm peroxide content (as H2O2),<200 μM carbonyls,<100 μS conductivity
Note: Purified to reduce levels of unwanted peroxides, carbonyls, metals, and salts. To preserve the low peroxide content of the preparation, always remove aliquots of detergent from the vial with a syringe.

Formula variant

C34H62O11, for n = 10

Preparation Note

Working concentration: 1-5 mM
CMC**: approximately 0.2 mM at +25°C
** CMC: critical micellar concentration

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Certificate of Analysis

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Certificate of Origin

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