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SCC119

Sigma-Aldrich

ImKC Immortalized Mouse Kupffer Cell Line

The ImKC Murine Immortalized Kupffer Cell Line can be used to develop in vitro model cell systems to advance the study of liver injury, function, and disease.

Synonym(s):
ImKC Cell Line
eCl@ss:
32011203
NACRES:
NA.81

Quality Level

biological source

mouse

application(s)

cell based assay: suitable
cell culture | mammalian: suitable

shipped in

ambient

Related Categories

General description

Kupffer cells (KC), also known as stellate macrophages, are liver macrophages that reside in the lumen of the liver sinusoids. Kupffer cells play a critical role in maintaining liver functions including the removal of pathogens & foreign materials, antigen presentation and modulating innate immune responses. In vitro studies of Kupffer cells have been hindered by the difficulty in isolating sufficient number of primary cells and the limited lifespan of the cells in culture.
ImKC is a cytokine-producing immortalized Kupffer cell line that was established from transgenic mice expressing the thermolabile mutant tsA58 of the Simian virus 40 large T antigen under the control of the H-2kb promoter. Primary KC were obtained using a three step procedure: liver perfusion, centrifugal elutriation, and sorting for F4/80+ cells. ImKC were identified within the small-intermediate population of KC that maintained stable expression of F4/80, and the surface antigens CD11b, CD14 and TLR4.

Cell Line Description

Macrophage Cells

Application

This product is intended for sale and sold solely for internal non-commercial research use per the terms of the “Restricted Use Agreement” as detailed in the product documentation. For information regarding any other uses, please contact licensing@emdmillipore.com.
Research Sub Category
Liver Physiology
Research Category
Inflammation & Immunology

Metabolism

Quality

• Each vial contains ≥ 1X106 viable cells.
• Cells tested negative for infectious diseases by a Mouse/Rat Comprehensive CLEAR panel PCR panel by Charles River Animal Diagnostic Services.
• Cells tested negative for mycoplasma contamination

Storage and Stability

Store in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

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