MABS2320

Anti-DNP Antibody, clone 1D10.4

• Synonym(s): DNP, Dinitrophenol
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: mouse
• Quality Level: 200
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1D10.4, monoclonal
• mol wt: observed mol wt ~66 kDa
• purified by: using protein G
• species reactivity (predicted by homology): all
• packaging: antibody small pack of 100
• technique(s): ELISA: suitable; western blot: suitable
• isotype: IgG2bκ
• UniProt accession no.: n/a
• storage temp.: 2-8°C

Description

Specificity

Clone 1D10.4 is a mouse monoclonal antibody that detects Dinitrophenol (DNP).

Immunogen

KLH-conjugated Dinitrophenol (DNP).

Application

Quality Control Testing

Evaluated by Western Blotting with DNP conjugated to BSA.

Western Blotting Analysis: A 1:500 dilution of this antibody detected DNP-conjugated BSA.

Tested Applications

ELISA Analysis: A representative lot of this antibody detected DNP in ELISA application.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Dinitrophenols (DNPs) are highly toxic synthetic chemical compounds, classified as a mitochondrial uncouplers, as they disrupt the process of oxidative phosphorylation in mitochondria, leading to a significant increase in metabolic rate and heat production. DNPs can prevent adenosine triphosphate (ATP) synthesis, but permit electron transfer to proceed. Beside their commercial use as fungicides, herbicides, and insecticides, DNPs are useful tools to study cellular bioenergetics and the mechanisms of thermogenesis. It can be used as a reactant for catalytic reduction reactions, activation of carboxylic acids by converting them into dinitrophenyl (DNP) esters among others.

Physical form

Purified mouse monoclonal antibody IgG2b in PBS with 0.05% sodium azide.

Reconstitution

1 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommended storage: +2°C to +8°C.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable