Recommended Products
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies, primary antibodies
clone
Y6206, monoclonal
mol wt
calculated mol wt 63.3 kDa
observed mol wt ~62 kDa
purified by
using protein G
species reactivity
mouse, human
packaging
antibody small pack of 100 μg
technique(s)
western blot: suitable
isotype
IgG2bκ
epitope sequence
N-terminal half
Protein ID accession no.
UniProt accession no.
shipped in
ambient
target post-translational modification
unmodified
Gene Information
human ... Cnot6(57472)
General description
CCR4-NOT transcription complex subunit 6 (UniProt: Q9ULM6; also known as EC:3.1.13.4, CCR4 carbon catabolite repression 4-like, Carbon catabolite repressor protein 4 homolog, Cytoplasmic deadenylase) is encoded by the Cnot6 (also known as Ccr4, Kiaa1194) gene (Gene ID: 57472) in human species. CNOT6, a catalytic component of the CCR4-NOT complex with in mammals. This complex is ubiquitously expressed and contains at least ten subunits. Subunits CNOT6, CNOT7, and CNOT8 display deadenylase activity and CNOT1, 2, 3, 9, 10, and 11 have regulatory NOT modules. CCR4-NOT complex is reported to limit expression of specific genes through deadenylation of mRNA poly(A) tails, enabling positive selection. CNOT6 is detected both in the nucleus and in cytoplasm. It also serves as a poly(A) nuclease with 3′-5′ RNase activity. It has been linked to multiple cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation, and general transcription regulation. The deadenylase activity of CCR4-NOT complex has also been implicated in the control of obesity and energy metabolism. In the liver, the deadenylase activity is recruited to the 3 -end of mRNAs of energy metabolism-associated genes and shortens their lifespans and reduces their expression levels. Subunits CNOT 6, 7, and 8 are also shown to participate in the regulation of induced pluripotent stem cell generation and CNOT1, 2, and 3 are involved in maintenance and self-renewal of embryonic stem cells. (Ref.: Ito-Kureha, T., et al. (2020). Nat. Commun. 11; 6169; Zukeran, A., et al. (2016). Biochem. Biophys, Res. Commun. 474; 233-239; Takahashi, A., et al. (2015). Cell Reports 13, 2756-2767).
Specificity
Clone Y6202 is a mouse monoclonal antibody that detects CCR4-NOT transcription complex subunit 6 (CNOT6). It targets an epitope within the N-terminal half.
Immunogen
A recombinant fragment corresponding to 50 amino acids from the N-terminal half of murine CNOT6 fused with baculovirus gp64 protein.
Application
Quality Control Testing
Evaluated by Western Blotting in HepG2 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected CNOT6 in HepG2 cell lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected CNOT6 in lysates from Mouse embryonic stem cells and DLD-1 cells.
Western Blotting Analysis: A representative lot detected CNOT6 in Western Blotting applications (Ito-Kureha, T., et al. (2020). Nat Commun.11(1):6169; Otsuka, H., et al. (2020). Biochimie.;174:49-56; Mostafa, D., et al. (2020). RNA Biol.;17(3):403-416; Zukeran, A., et al. (2016). Biochem Biophys Res Commun. 474(2):233-239; Takahashi, A., et al. (2015). Cell Rep. 13(12):2756-67).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in HepG2 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected CNOT6 in HepG2 cell lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected CNOT6 in lysates from Mouse embryonic stem cells and DLD-1 cells.
Western Blotting Analysis: A representative lot detected CNOT6 in Western Blotting applications (Ito-Kureha, T., et al. (2020). Nat Commun.11(1):6169; Otsuka, H., et al. (2020). Biochimie.;174:49-56; Mostafa, D., et al. (2020). RNA Biol.;17(3):403-416; Zukeran, A., et al. (2016). Biochem Biophys Res Commun. 474(2):233-239; Takahashi, A., et al. (2015). Cell Rep. 13(12):2756-67).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-CNOT6, clone Y6206, Cat. No. MABE1878, is a mouse monoclonal antibody that detects CCR4-NOT transcription complex subunit 6 (CNOT6) and is tested for use in Western Blotting.
Physical form
Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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