MABC39

Anti-LAMP-1 (CD107a) Antibody, clone 1D4B

clone 1D4B, from rat

• Synonym(s): lysosomal-associated membrane protein 1, lysosome-associated membrane glycoprotein 1, Lysosome-associated membrane protein 1, CD107 antigen-like family member A, CD107a antigen
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rat
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 1D4B, monoclonal
• species reactivity: mouse
• technique(s): flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotype: IgG2aκ
• NCBI accession no.: NP_034814
• UniProt accession no.: P11438
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: mouse ... Lamp1(16783)

Description

General description

Lysosome-associated membrane protein 1 (LAMP-1) is a highly glycosolated, single-pass type 1 membrane protein belonging to the lysosome- associated membrane protein family; a group of proteins that make up the majority of the lysosomal membrane and are considered crucial mediators for cellular adhesion by interacting with carbohydrate receptors. LAMP-1 functions to traffic carbohydrate ligands to selectins. It is also thought to play a role in tumor cell metastasis.

Immunogen

NIH/3T3 plasma membrane fraction of mouse LAMP-1.

Application

Detect LAMP-1 (CD107a) using this Anti-LAMP-1 (CD107a) Antibody, clone 1D4B validated for use in WB, IP, IC, IH, FC.

Immunoprecipitation Analysis: A previous lot was used by an independent laboratory in IP (Chen, 1985).

Immunocytochemistry Analysis: A previous lot was used by an independent laboratory in IC (Chen, 1985).

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

Tumor Markers

Quality

Evaluated by Western Blot in NIH/3T3 cell lysate.

Western Blot Analysis: 1:50,000 dilution of this antibody detected LAMP-1 in 10 µg of NIH/3T3 cell lysate.

Target description

~ 100 kDa observed. The calculated molecular weight of this protein is 44 kDa The protein is heavily glycosolated and therefore its molecular weight is typically noted from 95-115 kDa

Physical form

Format: Purified

Protein G

Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
NIH/3T3 cell lysate

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable