ABE279

Anti-HIF-1α Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): ARNT interacting protein, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, HIF-1 alpha, HIF1 alpha, Member of PAS protein 1, hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor), hypoxia-inducible fact
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human
• technique(s): western blot: suitable
• NCBI accession no.: NP_001521
• UniProt accession no.: Q16665
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... HIF1A(3091)

Description

General description

Cell growth and viability is compromised by oxygen deprivation (hypoxia). Hypoxia-inducible factors, including HIF-1α, HIF-1β (also designated Arnt 1), EPAS-1 (also designated HIF-2α) and HIF-3α, induce glycolysis, erythropoiesis and angiogenesis in order to restore oxygen homeostasis. Hypoxia-inducible factors are members of the Per-Arnt-Sim (PAS) domain transcription factor family. In response to hypoxia, HIF-1α is upregulated and forms a heterodimer with Arnt 1 to form the HIF-1 complex. The HIF-1 complex recognizes and binds to the hypoxia responsive element (HRE) of hypoxia-inducible genes, thereby activating transcription. Hypoxia-inducible expression of some genes such as Glut-1, p53, p21 or Bcl-2, is HIF-1α dependent, whereas expression of others, such as p27, GADD 153 or HO-1, is HIF-1α independent. EPAS-1 and HIF-3α have also been shown to form heterodimeric complexes with Arnt 1 in response to hypoxia.

Immunogen

KLH-conjugated linear peptide corresponding to human HIF-1α.

Application

Anti-HIF-1α Antibody is a Rabbit Polyclonal Antibody for detection of HIF-1 alpha also known as ARNT interacting protein or HIF-1 alpha & has been validated in WB.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Quality

Evaluated by Western Blot in cobalt chloride untreated and treated MCF-7 cell lysates.

Western Blot Analysis: 1 µg/mL of this antibody detected HIF-1α on 10 µg of cobalt chloride untreated and treated MCF-7 cell lysates.

Target description

~100 kDa observed

Linkage

Replaces: 04-1006

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Cobalt chloride untreated and treated MCF-7 cell lysates

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable