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400052

Millipore

Hygromycin B, Streptomyces sp., Sterile-Filtered Solution in PBS, Cell Culture-Tested - CAS 31282-04-9 - Calbiochem

Hygromycin B is an aminoglycoside antibiotic that inhibits the growth of prokaryotic and eukaryotic microorganisms and mammalian cells. Inhibits protein synthesis by interfering with the translocation of the 70S ribosome and causes misreading of mRNA.

Synonym(s):
Hygromycin B, Streptomyces sp., Sterile-Filtered Solution in PBS, Cell Culture-Tested - CAS 31282-04-9 - Calbiochem
Empirical Formula (Hill Notation):
C20H37N3O13
CAS Number:
Molecular Weight:
527.52
MDL number:

Quality Level

assay

≥85% (HPLC and TLC)

form

liquid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

shipped in

ambient

storage temp.

2-8°C

InChI

1S/C20H37N3O13/c1-23-7-2-5(21)9(26)15(10(7)27)33-19-17-16(11(28)8(4-25)32-19)35-20(36-17)18(31)13(30)12(29)14(34-20)6(22)3-24/h5-19,23-31H,2-4,21-22H2,1H3/t5-,6+,7+,8-,9+,10-,11+,12-,13-,14-,15-,16+,17+,18-,19+,20?/m1/s1

InChI key

GRRNUXAQVGOGFE-KPBUCVLVSA-N

General description

Sterile liquid formulation of Cat. No. 400050.
Hygromycin B is an aminoglycoside antibiotic that inhibits the growth of prokaryotic and eukaryotic microorganisms and mammalian cells. Inhibits protein synthesis by interfering with the translocation of the 70S ribosome and causes misreading of mRNA. Resistance to hygromycin B is conferred by a gene whose product, hygromycin B phospho-transferase that phosphorylates and inactivates the drug. Hygromycin B has been used to select mutants carrying the hygromycin B resistance gene in a wide variety of cells including bacterial, protozoal, yeast, fungal, plant, and mammalian cells. Typical concentrations used for mammalian cell selection are between 0.05 to 1 mg/ml. The appropriate concentration for use must be determined empirically for each cell type.

Packaging

20, 5, 50 ml in Alu drum

Application

Hygromycin B is an aminoglycoside antibiotic that inhibits the growth of prokaryotic and eukaryotic microorganisms and mammalian cells. Inhibits protein synthesis by interfering with the translocation of the 70S ribosome and causes misreading of mRNA.

Biochem/physiol Actions

Potency: ≥1000 U/mg

Warning

Toxicity: Highly Toxic & Carcinogenic / Teratogenic (I)

Physical form

50 mg/ml active antibiotic in sterile-filtered PBS, pH 7.2.

Other Notes

Sugimoto, K., et al. 1994. Plant J.5, 863.
Lee, M.G., and van der Ploeg, L.H. 1991. Gene105, 255.
Salauze, D., et al. 1990. Antimicrob. Agents Chemother.34, 1915.
Cullen, D., et al. 1987. Gene57, 21.
Rao, S.N., et al. 1983. Antimicrob. Agents Chemother.24, 689.
Santerre, R.F., et al. 1984. Gene30, 147.
Gonzalez, A., et al. 1978. Biochim. Biophys. Acta521, 459.
Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Skull and crossbones

Signal Word

Danger

Hazard Statements

Hazard Classifications

Acute Tox. 1 Inhalation - Acute Tox. 3 Oral - Acute Tox. 4 Dermal

Storage Class Code

6.1B - Non-combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Quality

Enter Lot Number to search for Certificate of Quality (COQ).

Sarah L Teakel et al.
Biochemical and biophysical research communications, 524(1), 64-69 (2020-01-26)
PGRMC1 is a protein from the MAPR family with a range of cellular functions. PGRMC1 has been described to play a role in fertility, neuroprotection, steroidogenesis, membrane trafficking and in cancer cell biology. PGRMC1 represents a likely key regulator of

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