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Key Documents

14-731-M

Sigma-Aldrich

CaM Kinase Iδ Protein, active, 20 µg

Synonym(s):

CaMKI-delta, Calcium/calmodulin-dependent protein kinase type 1D

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About This Item

UNSPSC Code:
12352204
eCl@ss:
32160405

biological source

human

Quality Level

100
300

mol wt

Mw 46.7 kDa

manufacturer/tradename

Upstate®

technique(s)

activity assay: suitable

NCBI accession no.

UniProt accession no.

General description

N-terminal 6His-tagged, recombinant, human, full length CaM Kinase Iδ.
Recombinant human protein expressed by baculovirus in Sf21 cells.

Application

Active, N-terminal 6His-tagged, recombinant, human, full length CaM Kinase Iδ. For use in Enzyme Assays.
Research Category
Metabolism|Inflammation & Immunology
Research Sub Category
Obesity|Metabolic Disorders|Osteoporosis|Arthritis

Biochem/physiol Actions

  • Protein Target: CaMKIΔ
  • Target Sub-Family:CAMK

Quality

Routinely evaluated by phosphorylation of 150μM KKLRRTLSVA.

Physical form

Format: Ni2+/NTA-agarose
Presented as a frozen liquid in 50mM Tris-HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol.

Storage and Stability

Stable for 6 months at -70°C from date of shipment. Upon first use, rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrog

Other Notes

Concentration:For concentration, please refer to the Certificate of Analysis for individual lots of this enzyme.
For Specific Activity data, please refer to the Certificate of Analysis for individual lots of this enzyme.
For purity information, please refer to the Certificate of Analysis for individual lots of this enzyme.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial

pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Skin Sens. 1

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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