Skip to Content
MilliporeSigma
All Photos(1)

Key Documents

09-078

Sigma-Aldrich

Anti-EHMT1/GLP1 Antibody

Upstate®, from rabbit

Synonym(s):

G9a like protein, H3-K9-HMTase, Histone H3-K9 methyltransferase, Histone-lysine N-methyltransferase, H3 lysine-9 specific, EC 2.1.1.43, euchromatic histone methyltransferase 1, euchromatic histone-lysine N-methyltransferase 1, Eu-HMTase1, FLJ12879 1, FP1

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

manufacturer/tradename

Upstate®

technique(s)

western blot: suitable

isotype

IgG

NCBI accession no.

NM_012117.1

UniProt accession no.

Q9H9B1

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... EHMT1(79813)

General description

G9a-Like Protein 1 (GLP1), also known as Euchromatic histone lysine N-methyl-transferase 1 (EMHT1), is a histone methyltransferase that methylates Lys9 of histone H3. H3Lys9 methylation is a marker for epigenetic transcriptional repression. EMHT1/GLP1 is targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. During G0 phase, it contributes to silencing of Myc- and E2F-responsive genes, suggesting a role in G0/G1 transition in cell cycle. EMHT1/GLP1 is phosphorylated upon DNA damage, probably by ATM or ATR. Defects in EHMT1 result in severe mental retardation, hypotonia, and epileptic seizures.

Specificity

Recognizes EMHT1/GLP1

Immunogen

Epitope: a.a. 40-59
KLH conjugated synthetic peptide (KHTQDSARVNPQDGTNTLTR(C) corresponding to a.a. 40-59 of human EMHT1/GLP1.

Application

Research Category
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology

Histones
Use Anti-EHMT1/GLP1 Antibody (Rabbit Polyclonal Antibody) validated in WB to detect EHMT1/GLP1 also known as H3-K9-HMTase 5, Histone H3-K9 methyltransferase 5.

Quality

Routinely evaluated by immunoblot.

Target description

160 kda

Physical form

ImmunoAffinity Purified
Immunoaffinity purified rabbit IgG in PBS containing 0.1% sodium azide, 30% glycerol

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Dan Levy et al.
Nature immunology, 12(1), 29-36 (2010-12-07)
Signaling via the methylation of lysine residues in proteins has been linked to diverse biological and disease processes, yet the catalytic activity and substrate specificity of many human protein lysine methyltransferases (PKMTs) are unknown. We screened over 40 candidate PKMTs
Rui Wang et al.
Journal of the American Chemical Society, 135(3), 1048-1056 (2012-12-19)
Protein methyltransferases (PMTs) have emerged as important epigenetic regulators in myriad biological processes in both normal physiology and disease conditions. However, elucidating PMT-regulated epigenetic processes has been hampered by ambiguous knowledge about in vivo activities of individual PMTs particularly because
Coralie Poulard et al.
EMBO reports, 18(8), 1442-1459 (2017-06-16)
Like many transcription regulators, histone methyltransferases G9a and G9a-like protein (GLP) can act gene-specifically as coregulators, but mechanisms controlling this specificity are mostly unknown. We show that adjacent post-translational methylation and phosphorylation regulate binding of G9a and GLP to heterochromatin

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service