MDM2 is over-expressed in many tumors. Its principal function is the ubiquitination and degradation of p53 tumor suppressor protein. This monoclonal antibody also recognizes a peptide epitope around Thr-216 of murine MDM2 when Thr-216 is unphosphorylated. SMP14 also cross reacts with some cytokeratins (6, 14 & 16). This is only a problem when working with certain epithelial cells and not fibroblasts.
This antibody recognizes MDM2.
His-tagged recombinant protein corresponding to human MDM2.
Research Sub Category
RNA Metabolism & Binding Proteins
Ubiquitin & Ubiquitin Metabolism
Immunoprecipitation Analysis: A representative lot was used by an independent laboratory for this application. (Changgong, L., et al. (2002). Molecular and Cellular Biology. 22(21):7562-7571.)
Epigenetics & Nuclear Function
Anti-MDM2 Antibody, clone 3G9 is a Mouse Monoclonal Antibody for detection of MDM2 also known as Double minute 2 protein or Mdm2 p53 binding protein homolog & has been validated in WB, IP, ICC & IHC.
Evaluated by Western Blot in MCF-7 cell lysate.
Western Blot Analysis: 0.5 µg/ml of this antibody detected MDM2 on 10 µg of MCF-7 cell lysate.
~ 75 kDa
Protein G Purified
Purified mouse monoclonal IgGκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
MCF-7 cell lysate
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.