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SP®-2560 Capillary GC Column

L × I.D. 200 m × 0.25 mm, df 0.20 μm


Quality Level


fused silica


AOAC® 2012.13,Ce 1-h05,996.06
meets requirements for USP G5


≤25-250 °C temperature (isothermal or programmed)

Beta value



0.20 μm


gas chromatography (GC): suitable

L × I.D.

200 m × 0.25 mm

matrix active group

Non-bonded; poly(biscyanopropyl siloxane) phase

Featured Industry

Food and Beverages

column type

capillary highly polar

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General description

Application: This highly polar biscyanopropyl column was specifically designed for detailed separation of geometricpositional (cis/trans) isomers of fatty acid methyl esters (FAMEs). It is extremely effective for FAME isomer applications.
USP Code: This column meets USP G5 requirements.
  • Non-bonded
  • Poly(biscyanopropyl siloxane)
Temp. Limits:
  • Subambient to 250 °C (isothermal or programmed)

Legal Information

AOAC is a registered trademark of AOAC International
SP is a registered trademark of Sigma-Aldrich Co. LLC

Certificate of Analysis

Certificate of Origin

Svein A Mjøs
Journal of chromatography. A, 1100(2), 185-192 (2005-10-21)
The trans isomers of 5,8,11,14,17-eicosapentaenoic acid (EPA) and 4,7,10,13,16,19-docosahexaenoic acid (DHA) methyl esters were prepared by isomerisation with paratoluenesulfinic acid (PTSA) in dioxane. The isomers were fractionated by silver ion liquid chromatography with baseline resolution between the isomers with different...
Carla Ragonese et al.
Analytical chemistry, 81(13), 5561-5568 (2009-06-02)
The present research is focused on the evaluation of one 0.10 mm i.d. and two 0.25 mm i.d., ionic liquid (IL) stationary phase [1,9-di(3-vinyl-imidazolium) nonane bis(trifluoromethyl) sulfonyl imidate] columns, with lengths of 12 (the microbore capillary), 30 and 100 m...
Noelia Aldai et al.
Lipids, 47(8), 821-825 (2012-06-15)
Rumen metabolism (e.g., biohydrogenation) of dietary unsaturated fatty acids (FA) is one of the main reasons why ruminant fats tend to be highly saturated and contain many isomerized FA intermediates. The process by which long-chain (20- to 24-carbon FA) polyunsaturated...
Kazuaki Yoshinaga et al.
Journal of oleo science, 62(10), 781-788 (2013-10-04)
The gas chromatography-flame ionization detector equipped with a higher polarity column (i.e., SP-2560) has often been used for the quantification of trans-fatty acids in food. In particular, AOCS Ce 1h-05, the official method of the American Oil Chemists' Society (AOCS)...
Shiping Shao et al.
Se pu = Chinese journal of chromatography, 33(11), 1214-1218 (2016-03-05)
A method to determine docosahexenoic acid (DHA) in milk powder by gas chromatography was established. The milk powder samples were hydrolyzed with hydrochloric acid, extracted to get total fatty acids by Soxhlet extractor, then esterified with potassium hydroxide methanol solution...


200 meter GC Columns for Detailed Analysis of cis/trans FAME Isomers

The SP-2560/SLB-IL111 column pairing allows for comprehensive and accurate (qualitative and quantitative) determinations of saturated and trans fatty acids.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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