T8154

Trypan Blue solution

0.4%, liquid, sterile-filtered, suitable for cell culture

• Synonym(s): Blue cell viability dye
• Empirical Formula (Hill Notation): C₃₄H₂₄N₆Na₄O₁₄S₄
• CAS Number: 72-57-1
• Molecular Weight: 960.81
• Beilstein/REAXYS Number: 4360496
• MDL number: MFCD00003969
• UNSPSC Code: 12352207
• PubChem Substance ID: 57654704
• NACRES: NA.75

Properties

• sterility: sterile-filtered
• Quality Level: 200
• form: liquid
• storage condition: dry at room temperature
• concentration: 0.4%
• technique(s): cell culture | mammalian: suitable; tissue processing: suitable
• application(s): cell analysis
• shipped in: ambient
• SMILES string: [Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1N=Nc2c(O)c3c(N)cc(cc3cc2S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(N=Nc5c(O)c6c(N)cc(cc6cc5S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4
• InChI: 1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4
• InChI key: GLNADSQYFUSGOU-UHFFFAOYSA-J

Description

General description

Trypan Blue (TB), an anionic hydrophilic azo dye, crosses only the cell membranes of dead cells, thereby staining dead tissues/cells blue. It is used in quantitative microscopy for a dye exclusion staining technique to differentiate between live and dead mammalian cells.

Trypan blue is a dye used to distinguish between live and dead cells. It is a vital stain that is not absorbed by healthy viable cells, but stains cells with a damaged cell membrane. This way, only dead cells can be counted. The method is sometimes referred to as the dye exclusion method.

Application

Trypan Blue solution has been used:

• in cell viability assay to count viable and dead cells
• in rescue assay to count viable cells
• in trypan blue exclusion counting method to determine proliferation curve
• as the saline control and in the preparation of lipopolysaccharide (LPS) solution to confirm the success of LPS infusion
• in trypan blue dye exclusion test to determine gastric epithelial cells (GEC) viability
• to count GEC cells using a hemocytometer

Preparation Note

Prepared in 0.81% sodium chloride and 0.06% potassium phosphate, dibasic.

Safety Information

• pictograms: GHS08
• signalword: Danger
• hcodes: H350
• pcodes: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Hazard Classifications: Carc. 1B
• Storage Class: 6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type ABEK (EN14387) respirator filter